Source	Chinese Hamster Ovary cell line, CHO-derived porcine Tie-2 protein Ala23-Lys746, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala23
Predicted Molecular Mass	81 kDa
SDS-PAGE	94-105 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Porcine Tie-2 His-tag (Catalog # 10492-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag (Catalog # 623-AN) binds with an ED₅₀ of 0.3-2.7 ng/mL.

Recombinant Porcine Tie-2 His-tag Protein, CF Scientific Data Examples

	Recombinant Porcine Tie-2 His-tag Protein Binding Activity When Recombinant Porcine Tie-2 His-tag (10492-T2) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 His-tag (623-AN) binds with an ED₅₀ of 0.3-2.7 ng/mL.
	Recombinant Porcine Tie-2 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Porcine Tie-2 His-tag Protein (Catalog # 10492-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-105 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10492-T2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region (1). These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis (2). Porcine Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein that shares 94% sequence homology with human Tie-2 in the extracellular domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects (3).

Item	Value
References	Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159. Takakura, N. et al. (1998) Immunity 9:677. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names	CD202b, CD202b antigen, EC 2.7.10, EC 2.7.10.1, TEK, TEK tyrosine kinase, endothelial, TIE2CD202b, Tie-2, Tie2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, VMCM, VMCM1, angiopoietin-1 receptor, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multiple cutaneous and mucosal

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Recombinant Porcine Tie-2 His-tag Protein, CF

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