Recombinant Human Angiopoietin-2 Protein

Name: Recombinant Human Angiopoietin-2 Protein
Brand: R&D Systems
SKU: 623-AN
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 623-AN

Citations (45)

(4)

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Key Product Details

Source:	NS0
Accession #:	O15123
Structure / Form:	Oligomer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Angiopoietin-2 Protein

Product Specifications for Recombinant Human Angiopoietin-2 Protein

Source	Mouse myeloma cell line, NS0-derived human Angiopoietin-2 protein Tyr19-Phe496, with a C-terminal 6-His tag
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Tyr19
Predicted Molecular Mass	56 kDa
SDS-PAGE	66 kDa, reducing conditions
Activity	Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2. 0.2 μg/mL of Recombinant Human Angiopoietin‑2 significantly induces phosphorylation of human Tie-2. Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin‑2 (Catalog # 623-AN) binds with an ED₅₀ of 0.5-4 ng/mL.

Scientific Data Examples for Recombinant Human Angiopoietin-2 Protein

	Recombinant Human Angiopoietin-2 Protein Binding Activity When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds with an ED₅₀ of 0.5-4 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

623-AN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, CHAPS and PEG with BSA as a carrier protein and Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

623-AN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, CHAPS and PEG with Trehalose. *1 MG pack size is supplied as a 0.2 µm filtered solution in MOPS, NaCl, CHAPS and PEG.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *1 MG pack size is shipped with dry ice or equivalent. Upon receipt, store it immediately at -70 °C.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution. 1 month, 2 to 8 °C under sterile conditions after reconstitution.

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Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled‑coil domain (aa 166-248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275-495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65-66 kDa in size. Under non‑reducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers (3-6). Alternate splicing generates a short isoform that lacks 52 amino acids (aa) preceding the coiled‑coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang-1 is a potent Tie‑2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist (3, 8‑11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the up‑regulation of ICAM‑1 and VCAM‑1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is up‑regulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17-20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).

Item	Value
References	Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165. Murdoch, C. et al. (2007) J. Immunol. 178:7405. Maisonpierre, P.C. et al. (1997) Science 27:55. Kim, I. et al. (2000) J. Biol. Chem. 275:18550. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126. Gale, N.W. et al. (2002) Dev. Cell 3:411. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772. Kim, I. et al. (2001) Cardiovasc. Res. 49:872. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205. Cao, Y. et al. (2007) Cancer Res. 67:3835. Nasarre, P. et al. (2009) Cancer Res. 69:1324. Dellinger, M. et al. (2008) Dev. Biol. 319:309. Fiedler, U. et al. (2006) Nat. Med. 12:235. Koga, K. et al. (2001) Cancer Res. 61:6248. Etoh, T. et al. (2001) Cancer Res. 61:2145. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680. Hu, B. et al. (2006) Cancer Res. 66:775. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.
Entrez Gene IDs	285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names	AGPT2, ANG-2, ANG2, ANGPT2, Angiopoietin-2, Tie2-ligand, angiopoietin 2, angiopoietin-2B, angiopoietin-2a, angiopoitin 2

Citations for Recombinant Human Angiopoietin-2 Protein (45)

Showing 1 - 10 of 45 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Angiopoietin-2 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

NM Karabid et al. (2022), Angpt2/Tie2 autostimulatory loop controls tumorigenesis Embo Molecular Medicine, 0(0):e14364.
PMID: 35266635
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay

S Tan et al. (2022), TIE2-high cervical cancer cells promote tumor angiogenesis by upregulating TIE2 and VEGFR2 in endothelial cells Translational Oncology, 26(0):101539.
PMID: 36116242
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Kalfon et al. (2022), Gastric Cancer-Derived Extracellular Vesicles (EVs) Promote Angiogenesis via Angiopoietin-2 Oncogene, 14(12).
PMID: 35740619
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

HJ Zhou et al. (2021), Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model Nature Communications, 12(1):504.
PMID: 33495460
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Harel et al. (2020), Roles of miR-640 and Zinc Finger Protein 91 (ZFP91) in Angiopoietin-1-Induced In Vitro Angiogenesis Cells, 9(7).
PMID: 32630670
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Carvalheir et al. (2020), Angiopoietin-2 Promotes Inflammatory Activation in Monocytes of Systemic Sclerosis Patients International Journal of Molecular Sciences, 21(24).
PMID: 33333969
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Cañas-Gonz et al. (2020), Influence of Stress and Depression on the Immune System in Patients Evaluated in an Anti-aging Unit Front Psychol, 11(0):1844.
PMID: 32849086
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Kim et al. (2019), Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration Sci Adv, 5(2):eaau6732.
PMID: 30788433
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EA Thackaberr et al. (2019), Rapid Development of Glaucoma Via ITV Nonselective ANGPT 1/2 Antibody: A Potential Role for ANGPT/TIE2 Signaling in Primate Aqueous Humor Outflow Invest. Ophthalmol. Vis. Sci., 60(13):4097-4108.
PMID: 31574535
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SS Lavoie et al. (2018), Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities J. Immunol., 0(0).
PMID: 29686052
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Conroy et al. (2018), IL-8 associates with a pro-angiogenic and mesenchymal subtype in glioblastoma Oncotarget, 9(21):15721-15731.
PMID: 29644004
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Daly et al. (2018), Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor Sci Rep, 8(1):505.
PMID: 29323190
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S La Porta et al. (2018), Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis J. Clin. Invest., 0(0).
PMID: 29355844
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Souma et al. (2018), Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 29358379
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Yang et al. (2018), Angiopoietin-2 promotes osteogenic differentiation of thoracic ligamentum flavum cells via modulating the Notch signaling pathway PLoS ONE, 13(12):e0209300.
PMID: 30557327
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA

H Elamaa et al. (2018), Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina Elife, 7(0).
PMID: 30444491
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Wang et al. (2018), Angiopoietin-2 promotes extracellular matrix degradation in human degenerative nucleus pulposus cells Int. J. Mol. Med., 0(0).
PMID: 29568930
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

M Wang et al. (2018), Novel inhibitors of the cellular RAS components, Poricoic acids, target Smad3 phosphorylation and Wnt/Î²-catenin pathway against renal fibrosis Br. J. Pharmacol., 0(0).
PMID: 29679507
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Wang et al. (2017), The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration Lab. Invest., 0(0).
PMID: 28394321
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KA Bohn et al. (2017), Inhibition of VEGF and Angiopoietin-2 to Reduce Brain Metastases of Breast Cancer Burden Front Pharmacol, 8(0):193.
PMID: 28443023
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Morooka et al. (2017), Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling Circ. Res, 0(0).
PMID: 28179430
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Kloepper et al. (2016), Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival Proc Natl Acad Sci USA, 113(16):4476-81.
PMID: 27044098
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Tie1 controls angiopoietin function in vascular remodeling and inflammation J Clin Invest, 0(0).
PMID: 27548530
Species: Human
Sample Types: Protein
Applications: Bioassay

Binyam Bezabeh (2016), Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties MAbs, 0(0):0.
PMID: 27981887
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson Chan (2016), Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells PLoS ONE, 11(6):e0156994.
PMID: 27304216
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1 Sci Adv, 2(4):e1501290.
PMID: 27757426
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

J Rautiola et al. (2016), Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma PLoS ONE, 11(4):e0153745.
PMID: 27100185
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release PLoS ONE, 11(9):e0163140.
PMID: 27632174
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hakanpaa L et al. (2015), Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 6(0):5962.
PMID: 25635707
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Brunckhorst M et al. (2014), Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment. Am J Pathol, 184(8):2285-96.
PMID: 25043619
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Haddad L et al. (2014), Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils. PLoS ONE, 9(2):e88980.
PMID: 24563688
Species: Human
Sample Types: Protein
Applications: Standard

Keats E et al. (2014), Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells, 32(6):1649-60.
PMID: 24496952
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Brindle et al. (2013), Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display. J Biol Chem, 288(46):33205-12.
PMID: 24106271
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yu et al. (2013), Structural basis for angiopoietin-1-mediated signaling initiation. Proc Natl Acad Sci U S A, 110(18):7205-10.
PMID: 23592718
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Reikvam H et al. (2010), Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 84(3):239-51.
PMID: 19922462
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Brunckhorst MK et al. (2010), Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res., 70(18):7283-93.
PMID: 20823154
Species: N/A
Sample Types: N/A
Applications: Quantum Dot

Soman C et al. (2009), Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation. Nanomedicine, 5(4):402-9.
PMID: 19523417
Species: N/A
Sample Types: N/A
Applications: Quantum Dot

Soman CP et al. (2008), Quantum dot self-assembly for protein detection with sub-picomolar sensitivity. Langmuir, 24(8):4399-404.
PMID: 18335969
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Saharinen P et al. (2008), Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat. Cell Biol., 10(5):527-37.
PMID: 18425119
Species: N/A
Sample Types: N/A
Applications: ELISA (Control)

Vart RJ et al. (2007), Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells. Cancer Res., 67(9):4042-51.
PMID: 17483315
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Rao S et al. (2007), Obligatory participation of macrophages in an angiopoietin 2-mediated cell death switch. Development, 134(24):4449-58.
PMID: 18039971
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: In Vivo
Applications: In Vivo

Xu F et al. (2005), Intraovarian actions of anti-angiogenic agents disrupt periovulatory events during the menstrual cycle in monkeys. Contraception, 71(4):239-48.
PMID: 15792643
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Danet GH et al. (2003), Expansion of human SCID-repopulating cells under hypoxic conditions. J. Clin. Invest., 112(1):126-35.
PMID: 12840067
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wu X et al. (0), Angiopoietin-2 as a Biomarker and Target for Immune Checkpoint Therapy. Cancer Immunol Re5(1):17-28.
PMID: 28003187
Species: Human
Sample Types: Cell Culture Supernates

Jenny Zhou H et al. (0), Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Me22(9):1033-1042.
PMID: 27548575

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Recombinant Human Angiopoietin-2 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 07/27/2020
Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 05/20/2020
Recombinant Human Angiopoietin-2 Protein

Name: Zoia Levashova

Application: Immunoassay Standard

Verified Customer | Posted 11/26/2018

The recombinant human Angiopoietin-2 protein was used as the calibration reagent in MSD assay with biotinylated MAB098 (capture) and Sulfo-Tag labeled MAB0984 (detection) antibodies.
Recombinant Human Angiopoietin-2 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 11/16/2017

The recombinant ANG-2 was used to create a standard ELISA curve.

Recombinant Human Angiopoietin-2 Protein

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Formulation, Preparation and Storage

Carrier Free

623-AN

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623-AN/CF

Carrier Free

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Background: Angiopoietin-2

Citations for Recombinant Human Angiopoietin-2 Protein (45)

Customer Reviews for Recombinant Human Angiopoietin-2 Protein (4)

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Recombinant Human Angiopoietin-2 Protein

Recombinant Human Angiopoietin-2 Protein

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