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Recombinant Mouse Tie-2 His-tag Protein, CF

Catalog # 10487-T2 | R&D Systems, Inc. a Bio-Techne Brand
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10487-T2-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Tie-2 protein
Ala23-Lys744, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

81 kDa

SDS-PAGE

96-107 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Tie-2 His-tag (Catalog # 10487-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag  (Catalog # 623-AN) binds with an ED50 of 0.4-3.6 ng/mL.

Scientific Data Images for Recombinant Mouse Tie-2 His-tag Protein, CF

Recombinant Mouse Tie-2 His-tag Protein Binding Activity

Recombinant Mouse Tie-2 His-tag Protein Binding Activity

When Recombinant Mouse Tie-2 His-tag (10487-T2) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED50 of 0.4-3.6 ng/mL.
Recombinant Mouse Tie-2 His-tag Protein SDS-PAGE

Recombinant Mouse Tie-2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Tie-2 His-tag Protein (Catalog # 10487-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-110 kDa.

Formulation, Preparation and Storage

10487-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.

References

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Gene Symbol

TEK

UniProt

Additional Tie-2 Products

Product Documents for Recombinant Mouse Tie-2 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Tie-2 His-tag Protein, CF

For research use only

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