Product Specifications for Recombinant Human Tie-2 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived human Tie-2 protein Ala23-Lys745, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
98-111 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 His-tag (Catalog # 10457-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag (Catalog # 623-AN) binds with an ED50 of 0.5-4 ng/mL.
Scientific Data Examples for Recombinant Human Tie-2 His-tag Protein, CF
Recombinant Human Tie-2 His-tag Protein Binding Activity
When Recombinant Human Tie-2 His-tag (10457-TI) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED50 of 0.5-4 ng/mL.
Recombinant Human Tie-2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Tie-2 His-tag Protein (Catalog # 10457-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 98-111 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-2 cDNA encodes a 1124 amino acid (aa) precursor protein with an 18 aa putative signal peptide, a 727 aa extracellular domain (ECD) and a 354 aa cytoplasmic domain. Human Tie-2 ECD shares 90% aa identity with mouse and rat Tie-2. Two ligands, angiopoietin1 (Ang1) and angiopoietin2 (Ang2), which bind Tie-2 with high affinity, have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to over-express Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
Takakura, N. et al. (1998) Immunity 9:677.
Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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