Recombinant Human Angiopoietin-like 3 Protein, CF

Name: Recombinant Human Angiopoietin-like 3 Protein, CF
Brand: R&D Systems
SKU: 3829-AN
Rating: 5 (1 reviews)

Catalog # 3829-AN | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (2)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q9Y5C1
Structure / Form:	Oligomer
Applications:	Bioactivity, Inhibition Activity

View Full Technical Details for Recombinant Human Angiopoietin-like 3 Protein, CF

Product Specifications for Recombinant Human Angiopoietin-like 3 Protein, CF

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Angiopoietin-like Protein 3/ANGPTL3 protein Ser17-Glu460, with a C-terminal 10-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser17
Predicted Molecular Mass	53.2 kDa
SDS-PAGE	54-59 kDa and 28-30 kDa, reducing conditions
Activity	Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 40-200 ng/mL in the presence of a cross-linking Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC₅₀ value under conditions in which Recombinant Human Lipoprotein Lipase/LPL Protein, CF (Catalog # 9888-LL) and p-nitrophenyl butyrate are present in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is <15 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3829-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1-3). Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do proteolytically separated N- and C-terminal fragments containing the coiled-coil domain and fibrinogen-like domains (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (7, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver, also promotes the expansion of hematopoietic stem cells (16). Mature human ANGPTL3 shares 24%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 5, 6, and 7. It shares 77% aa sequence identity with mouse ANGPTL3.

Item	Value
References	Li, C. (2006) Curr. Opin. Lipidol. 17:152. Oike, Y. et al. (2004) Int. J. Hematol. 80:21. Kersten, S. (2005) Biochem. Soc. Trans. 33:1059. Conklin, D. et al. (1999) Genomics 62:477. Ge, H. et al. (2005) J. Lipid Res. 46:1484. Ono, M. et al. (2003) J. Biol. Chem. 278:41804. Koishi, R. et al. (2002) Nat. Genet. 30:151. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366. Koster, A. et al. (2005) Endocrinology 146:4943. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553. Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Entrez Gene IDs	27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names	AGNPT5, ANGPT5angiopoietin-5, ANGPTL3, Ang-5, Angiopoietin-5, Angiopoietin-like Protein 3, FHBL2, angiopoietin 5, angiopoietin-like 3, angiopoietin-related protein 3

Citations for Recombinant Human Angiopoietin-like 3 Protein, CF (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Angiopoietin-like 3 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Aerts-Kaya F et al. (2020-01-07), SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential. Biol Blood Marrow Transplant, 2020-026(4):634-642.
PMID: 31917271
Species: Human
Sample Types: Plasma
Applications: Bioassay

Aivar Lookene (2016-11-14), Lipoprotein lipase activity and interactions studied in human plasma by isothermal titration calorimetry J. Lipid Res., 2016-10(0).
PMID: 27845686

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Customer Reviews for Recombinant Human Angiopoietin-like 3 Protein, CF (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human Angiopoietin-like 3 Protein, CF

Name: Anonymous

Application: Cell Adhesion

Verified Customer | Posted 10/21/2016

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents for Recombinant Human Angiopoietin-like 3 Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Human Angiopoietin-like 3 Protein, CF

Catalog # 3829-AN | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Human Angiopoietin-like 3 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Angiopoietin-like Protein 3/ANGPTL3

Citations for Recombinant Human Angiopoietin-like 3 Protein, CF (2)

Customer Reviews for Recombinant Human Angiopoietin-like 3 Protein, CF (1)

Customer Images

Recombinant Human Angiopoietin-like 3 Protein, CF

Product Documents for Recombinant Human Angiopoietin-like 3 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected