Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Name: Recombinant Mouse Flt-3 Ligand/FLT3L Protein
Brand: R&D Systems
SKU: 427-FL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 427-FL

Citations (86)

Product Datasheet / COA / SDS

Earn double rewards for reviews

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P49772.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Complete Your Research

Product Specifications for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Source	Mouse myeloma cell line, NS0-derived mouse Flt-3 Ligand/FLT3L protein Gly27-Arg188
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly27
Predicted Molecular Mass	18 kDa
SDS-PAGE	Multiple bands between 18-32 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED₅₀ for this effect is typically 0.4-2.4 ng/mL.

Scientific Data Examples for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

	Recombinant Mouse Flt-3 Ligand/FLT3L Protein Bioactivity Recombinant Mouse Flt-3 Ligand/FLT3L (Catalog # 427-FL) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED₅₀ for this effect is 0.4-2.4 ng/mL.
	Recombinant Mouse Flt-3 Ligand/FLT3L Protein SDS-PAGE 1 μg/lane of Recombinant Mouse Flt-3 Ligand/FLT3L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. Multiple bands are due to variable glycosylation of the protein.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

427-FL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

427-FL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 50 µg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. The mouse and human Flt-3 Ligand proteins show cross-species activity (4, 5, 7). Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various mouse and human tissues. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). Additionally, Flt-3 Ligand synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). Flt-3 Ligand also cooperates with IL‑2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL‑11 to induce terminal B cell maturation (1, 11). Animal studies also show that Flt-3 Ligand reduces the severity of experimentally induced allergic inflammation (12).

Item	Value
References	Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532. Hannum, C. et al. (1994) Nature 368:643. Lyman, S.D. et al. (1993) Cell 75:1157. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486. Lyman, S.D. et al. (1994) Blood 83:2795. McClanahan, T. et al. (1996) Blood 88:3371. Lyman, S.D. et al. (1995) Oncogene 10:149. Diener, K.R. et al. (2008) Exp. Hematol. 36:51. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.
Long Name	fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs	2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names	FL, FLG3L, FLT3LG, Flt-3 Ligand, Flt3 ligand, Flt3L, SL cytokine, fms-related tyrosine kinase 3 ligand

Citations for Recombinant Mouse Flt-3 Ligand/FLT3L Protein (86)

Showing 1 - 10 of 86 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Flt-3 Ligand/FLT3L Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Jeong et al. (2022), BAP1 shapes the bone marrow niche for lymphopoiesis by fine-tuning epigenetic profiles in endosteal mesenchymal stromal cells Cell Death and Differentiation, 0(0).
PMID: 35473985
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Hayashi et al. (2022), MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis Cell Reports, 39(6):110805.
PMID: 35545056
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Uslu et al. (2022), Competitive inhibition of IL-2/IL-2R has a dual effect on HSC ex vivo expansion and IL-2R (CD25) content International immunopharmacology, 110(0):109035.
PMID: 35834953
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

V Tereshchen et al. (2021), The murine DCs transfected with DNA-plasmid encoding CCR9 demonstrate the increased migration to CCL25 and thymic cells in vitro and to the thymus in vivo Cytokine, 142(0):155473.
PMID: 33647585
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

N Ohashi et al. (2021), GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS Scientific Reports, 11(1):12803.
PMID: 34140581
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Lu et al. (2021), Declined miR-181a-5p expression is associated with impaired natural killer cell development and function with aging Aging Cell, 0(0):e13353.
PMID: 33780118
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Yamamoto et al. (2021), A histone modifier, ASXL1, interacts with NONO and is involved in paraspeckle formation in hematopoietic cells Cell Reports, 36(8):109576.
PMID: 34433054
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

F Herbst et al. (2021), The balance between the intronic miR-342 and its host gene Evl determines hematopoietic cell fate decision Leukemia, 0(0).
PMID: 34021250
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SJ Gurczynski et al. (2021), Stem cell transplantation uncovers TDO-AHR regulation of lung dendritic cells in herpesvirus-induced pathology JCI Insight, 6(2).
PMID: 33491663
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

NE Papaioanno et al. (2021), Environmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice Nature Communications, 12(1):464.
PMID: 33469015
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SP Fahl et al. (2021), The E protein-TCF1 axis controls &gamma&delta Tï¿½cell development and effector fate Cell Reports, 34(5):108716.
PMID: 33535043
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Zhao et al. (2020), I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis Mediators Inflamm., 2020(0):6947482.
PMID: 32256194
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

L Garcia-Per et al. (2020), Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID Mol Ther Methods Clin Dev, 17(0):666-682.
PMID: 32322605
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Wada et al. (2020), Exï¿½Vivo Gene Therapy Treats Bone Complications of Mucopolysaccharidosis Type II Mouse Models through Bone Remodeling Reactivation Mol Ther Methods Clin Dev, 19(0):261-274.
PMID: 33102618
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

M Girotra et al. (2020), In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro Int J Mol Sci, 21(21).
PMID: 33153113
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

NV Bogert et al. (2020), A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus Sci Rep, 10(1):13740.
PMID: 32792615
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Hong et al. (2020), Authentication of Primary Murine Cell Lines by a Microfluidics-Based Lab-On-Chip System Biomedicines, 8(12).
PMID: 33317212
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

NV Bogert et al. (2020), A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus Sci Rep, 10(1):13740.
PMID: 32792615
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

LM Hillyer et al. (2019), Acutely malnourished weanling mice administered Flt3 ligand can support a cell-mediated inflammatory response Cytokine, 113(0):39-49.
PMID: 30539781
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Goossens et al. (2019), ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms Haematologica, 0(0).
PMID: 30679322
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

F Boscolo Se et al. (2019), Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy Cell Rep, 26(3):689-701.e6.
PMID: 30650360
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Vannini et al. (2019), The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance Cell Stem Cell, 24(3):405-418.e7.
PMID: 30849366
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

F Ribezzo et al. (2019), Rps14, Csnk1a1 and miRNA145/miRNA146a deficiency cooperate in the clinical phenotype and activation of the innate immune system in the 5q- syndrome Leukemia, 0(0).
PMID: 30651631
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Huang et al. (2019), Interleukin-33 regulates hematopoietic stem cell regeneration after radiation injury Stem Cell Res Ther, 10(1):123.
PMID: 30999922
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

ELY Chen et al. (2019), RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors Nat. Immunol., 0(0).
PMID: 31636466
Species: Mouse
Sample Types: Plasma
Applications: Multiplex Assay

JM Bertho et al. (2019), Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism Sci Rep, 9(1):19919.
PMID: 31882739
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Z Gong et al. (2019), Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development Cell Death Dis, 10(6):440.
PMID: 31165736
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

LC Cheung et al. (2018), New therapeutic opportunities from dissecting the pre-B leukemia bone marrow microenvironment Leukemia, 0(0).
PMID: 29740160
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Kanayama et al. (2018), Enhancement of immunomodulative effect of lactic acid bacteria on plasmacytoid dendritic cells with sucrose palmitate Sci Rep, 8(1):3147.
PMID: 29453437
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SH Kim et al. (2018), Anti-cancer activity of Angelica gigas by increasing immune response and stimulating natural killer and natural killer T cells BMC Complement Altern Med, 18(1):218.
PMID: 30021579
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

HT Fang et al. (2018), Global H3.3 dynamic deposition defines its bimodal role in cell fate transition Nat Commun, 9(1):1537.
PMID: 29670118
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

GH Nam et al. (2018), Combined Rho-kinase inhibition and immunogenic cell death triggers and propagates immunity against cancer Nat Commun, 9(1):2165.
PMID: 29867097
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

YW Lin et al. (2018), Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses Sci Rep, 8(1):12184.
PMID: 30111869
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Wünsche et al. (2018), Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs Cell Stem Cell, 23(1):132-146.e9.
PMID: 29979988
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JP Sasine et al. (2018), Wild-type Kras expands and exhausts hematopoietic stem cells JCI Insight, 3(11).
PMID: 29875320
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JP Sasine et al. (2018), Wild-type Kras expands and exhausts hematopoietic stem cells JCI Insight, 3(11).
PMID: 29875320
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

R Gonzalez-Q et al. (2018), Lupus acceleration by a MAVS-activating RNA virus requires endosomal TLR signaling and host genetic predisposition PLoS ONE, 13(9):e0203118.
PMID: 30199535
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SK Martin et al. (2018), mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis Sci Rep, 8(1):14501.
PMID: 30266921
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SK Tasian et al. (2018), Mutation-specific signaling profiles and kinase inhibitor sensitivities of juvenile myelomonocytic leukemia revealed by induced pluripotent stem cells Leukemia, 0(0).
PMID: 29884903
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Inoue et al. (2018), Arginine methylation controls the strength of ?c-family cytokine signaling in T cell maintenance Nat. Immunol., 19(11):1265-1276.
PMID: 30323341
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Huang et al. (2017), Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease Mol Ther Methods Clin Dev, 5(0):241-258.
PMID: 28603745
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Ma et al. (2017), Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs J. Exp. Med., 0(0).
PMID: 28416650
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Shukla et al. (2017), Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 14(5):531-538.
PMID: 28394335
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

MJ Smith et al. (2017), T cell progenitor therapy-facilitated thymopoiesis depends upon thymic input and continued thymic microenvironment interaction JCI Insight, 2(10).
PMID: 28515359
Species: Mouse
Sample Types: Whole Cells
Applications: Differentiation

Y Xiao et al. (2017), Macrophages and osteoclasts stem from a bipotent progenitor downstream of a macrophage/osteoclast/dendritic cell progenitor Blood Adv, 1(23):1993-2006.
PMID: 29296846
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

L Zhao et al. (2016), Orientation-specific RAG activity in chromosomal loop domains contributes to Tcrd V(D)J recombination during T cell development J. Exp. Med, 213(9):1921-36.
PMID: 27526713
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Kumar et al. (2016), Differential Cultivation of Francisella tularensis Induces Changes in the Immune Response to and Protective Efficacy of Whole Cell-Based Inactivated Vaccines Front Immunol, 7(0):677.
PMID: 28119692
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors Nat Commun, 7(0):13396.
PMID: 27869129
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins Nat. Med., 22(6):672-8.
PMID: 27135740
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Hepatitis C Virus Stimulates Murine CD8?-Like Dendritic Cells to Produce Type I Interferon in a TRIF-Dependent Manner PLoS Pathog, 12(7):e1005736.
PMID: 27385030
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), The non-canonical Wnt receptor Ryk regulates hematopoietic stem cell repopulation in part by controlling proliferation and apoptosis Cell Death Dis, 7(11):e2479.
PMID: 27882948
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ahmed S Elshikha (2016), Alpha 1 Antitrypsin Inhibits Dendritic Cell Activation and Attenuates Nephritis in a Mouse Model of Lupus PLoS ONE, 11(5):e0156583.
PMID: 27232337
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis PLoS ONE, 11(5):e0154630.
PMID: 27159398
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms Nat. Med, 0(0).
PMID: 27918563
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Niedzielska M et al. (2015), Selective Expression of the MAPK Phosphatase Dusp9/MKP-4 in Mouse Plasmacytoid Dendritic Cells and Regulation of IFN-beta Production. J Immunol, 195(4):1753-62.
PMID: 26170386
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Martinez-Lopez M et al. (2015), Batf3-dependent CD103+ dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice. Eur J Immunol, 45(1):119-29.
PMID: 25312824
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Flores M et al. (2015), FcgammaRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response. J Immunol, 194(9):4240-50.
PMID: 25821224
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2015), Lignin-rich enzyme lignin (LREL), a cellulase-treated lignin-carbohydrate derived from plants, activates myeloid dendritic cells via Toll-like receptor 4 (TLR4). J Biol Chem, 290(0):4410-21.
PMID: 25548274
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lee S et al. (2015), Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages. PLoS ONE, 10(7):e0132329.
PMID: 26147219
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay

Rommler F et al. (2015), Guanine-modified inhibitory oligonucleotides efficiently impair TLR7- and TLR9-mediated immune responses of human immune cells. PLoS ONE, 10(2):e0116703.
PMID: 25695778
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wallington-Beddoe C et al. (2014), Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression. Cancer Res, 74(10):2803-15.
PMID: 24686171
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Patnode M et al. (2014), Leukotriene B4 amplifies eosinophil accumulation in response to nematodes. J Exp Med, 211(7):1281-8.
PMID: 24889202
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Krutikov K et al. (2014), Ectopic TLX1 expression accelerates malignancies in mice deficient in DNA-PK. PLoS ONE, 9(2):e89649.
PMID: 24586935
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Coffey F et al. (2014), The TCR ligand-inducible expression of CD73 marks gammadelta lineage commitment and a metastable intermediate in effector specification. J Exp Med, 211(2):329-43.
PMID: 24493796
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Boettcher S et al. (2014), Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood, 124(9):1393-403.
PMID: 24990886
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Dai P et al. (2014), Modified vaccinia virus Ankara triggers type I IFN production in murine conventional dendritic cells via a cGAS/STING-mediated cytosolic DNA-sensing pathway. PLoS Pathog, 10(4):e1003989.
PMID: 24743339
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Treanor L et al. (2014), Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential. J Exp Med, 211(4):701-13.
PMID: 24687960
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Narendra S et al. (2014), Dendritic cells activated by double-stranded RNA induce arthritis via autocrine type I IFN signaling. J Leukoc Biol, 95(4):661-6.
PMID: 24304616
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Pereira C et al. (2013), Induction of a hemogenic program in mouse fibroblasts. Cell Stem Cell, 13(2):205-18.
PMID: 23770078
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Smith et al. (2013), The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci, 126(0):1366-80.
PMID: 23418353
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Manley et al. (2013), Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat. PLoS ONE, 8(4):e61789.
PMID: 23613937
Species: Cotton Rat
Sample Types: Whole Cells
Applications: Bioassay

Kim D et al. (2013), Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response. PLoS Pathog, 9(3):e1003233.
PMID: 23516365
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yamamizu K et al. (2013), Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 1(6):545-59.
PMID: 24371809
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Gatto D et al. (2013), The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells. Nat Immunol, 14(5):446-53.
PMID: 23502855
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Gao et al. (2013), Gata2 cis-element is required for hematopoietic stem cell generation in the mammalian embryo. J Exp Med, 210(13):2833-42.
PMID: 24297994
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Iborra S et al. (2012), The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice. J. Clin. Invest., 122(5):1628-43.
PMID: 22505455
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Shinohara ML et al. (2008), Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Immunity, 29(1):68-78.
PMID: 18619869
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rajesh</LastName><ForeNam D</Initial et al. (2007), Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 25(2):490-9.
PMID: 17284653
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Zelante T et al. (2007), IL-23 and the Th17 pathway promote inflammation and impair antifungal immune resistance. Eur. J. Immunol., 37(10):2695-706.
PMID: 17899546
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kared H et al. (2006), Jagged2-expressing hematopoietic progenitors promote regulatory T cell expansion in the periphery through notch signaling. Immunity, 25(5):823-34.
PMID: 17081781
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Braun BS et al. (2006), Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood, 108(6):2041-4.
PMID: 16720837
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Azhipa O et al. (2005), Comparative analysis of the fate of donor dendritic cells and B cells and their influence on alloreactive T cell responses under tacrolimus immunosuppression. Clin. Immunol., 114(2):199-209.
PMID: 15639654
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Miller MJ et al. (2004), T cell repertoire scanning is promoted by dynamic dendritic cell behavior and random T cell motility in the lymph node. Proc. Natl. Acad. Sci. U.S.A., 101(4):998-1003.
PMID: 14722354
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Asselin-Paturel C et al. (2003), Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody. J. Immunol., 171(12):6466-77.
PMID: 14662846
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Blumenthal RD et al. (2002), Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines. Clin. Cancer Res., 8(5):1301-9.
PMID: 12006552
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Developmet

Shih CC et al. (2000), A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 95(6):1957-66.
PMID: 10706861

Recombinant Mouse Flt-3 Ligand/FLT3L Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Scientific Data Examples for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Formulation, Preparation and Storage

Carrier Free

427-FL

Carrier

427-FL/CF

Carrier Free

Reconstitution Calculator

Background: Flt-3 Ligand/FLT3L

Citations for Recombinant Mouse Flt-3 Ligand/FLT3L Protein (86)

Product Documents for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected