Recombinant Mouse Flt-3 Ligand/FLT3L Protein Best Seller

Catalog # 427-FL | R&D Systems, Inc. a Bio-Techne Brand
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427-FL-005/CF
427-FL-025/CF
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427-FL-005
427-FL-025
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Key Product Details

Accession #

P49772.1

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Flt-3 Ligand/FLT3L protein
Gly27-Arg188

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly27

Predicted Molecular Mass

18 kDa

SDS-PAGE

Multiple bands between 18-32 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3.
The ED50 for this effect is typically 0.4-2.4 ng/mL.

Scientific Data Images for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Recombinant Mouse Flt-3 Ligand/FLT3L Protein Bioactivity

Recombinant Mouse Flt-3 Ligand/FLT3L Protein Bioactivity

Recombinant Mouse Flt-3 Ligand/FLT3L (Catalog # 427-FL) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.4-2.4 ng/mL.
Recombinant Mouse Flt-3 Ligand/FLT3L Protein SDS-PAGE

Recombinant Mouse Flt-3 Ligand/FLT3L Protein SDS-PAGE

1 μg/lane of Recombinant Mouse Flt-3 Ligand/FLT3L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. Multiple bands are due to variable glycosylation of the protein.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 427-FL
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 427-FL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. The mouse and human Flt-3 Ligand proteins show cross-species activity (4, 5, 7). Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various mouse and human tissues. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). Additionally, Flt-3 Ligand synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). Flt-3 Ligand also cooperates with IL‑2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL‑11 to induce terminal B cell maturation (1, 11). Animal studies also show that Flt-3 Ligand reduces the severity of experimentally induced allergic inflammation (12).

References

  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1993) Cell 75:1157.
  6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  7. Lyman, S.D. et al. (1994) Blood 83:2795.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Lyman, S.D. et al. (1995) Oncogene 10:149.
  10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Gene Symbol

FLT3LG

UniProt

P49772.1

Product Documents for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Download SDS 427-FL/CF (carrier free)

Product Specific Notices for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

For research use only

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