Recombinant Human Lipoprotein Lipase/LPL Protein, CF

Name: Recombinant Human Lipoprotein Lipase/LPL Protein, CF
Brand: R&D Systems
SKU: 9888-LL

Catalog # 9888-LL | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	P06858
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Lipoprotein Lipase/LPL Protein, CF

Technical Data
Citations (2) / Reviews

Product Specifications for Recombinant Human Lipoprotein Lipase/LPL Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human Lipoprotein Lipase/LPL protein Ala28-Gly475 with a C-terminal 6-His tag
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala28
Predicted Molecular Mass	51 kDa
SDS-PAGE	51 kDa
Activity	Measured by its ability to hydrolyze 4-Nitrophenyl butyrate. The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human Lipoprotein Lipase/LPL Protein, CF

	Recombinant Human Lipoprotein Lipase/LPL Protein Bioactivity Recombinant Human LPL activity can be inhibited by Recombinant Mouse ANGPTL3. Recombinant Mouse ANGPTL3 (Catalog # 9899-AN) dose dependently inhibits Recombinant Human LPL (Catalog # 9888-LL) activity with a ND₅₀of 2-10 µg/mL.
	Recombinant Human Lipoprotein Lipase/LPL Protein Enzyme Activity Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is measured by its ability to hydrolyze 4-Nitrophenyl butyrate.
	Recombinant Human Lipoprotein Lipase/LPL Protein SDS-PAGE 1 μg/lane of Recombinant Human Lipoprotein Lipase was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a band at 60 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9888-LL

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, CHAPS and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: Lipoprotein Lipase/LPL

Lipoprotein Lipase (LPL) is a rate-limiting enzyme responsible for the hydrolysis of triglycerides (1). LPL forms a non-covalent active homodimeric molecule (2). Monomeric LPL contains an N-terminal domain with the catalytic triad responsible for lipolysis and a 22-amino acid loop that serves as a cover for the catalytic site (3) in addition to a C-terminal domain that contains the region required for dimerization (4) as well as the primary heparin-binding domain that is important for lipoprotein binding. LPL is expressed in many tissues (5, 6) where it is synthesized in the ER of parenchymal cells and secreted to capillaries. LPL is highly controlled by regulatory factors such as apolipoproteins, angiopoietins, and hormones (7). LPL can be produced by macrophages and this expression is a critical event in the pathogenesis of atherosclerosis (8) in addition to contributing to the macrophage inflammatory response (9). Variants of LPL have been associated with altered risk of several diseases including coronary heart disease (10, 11), cerebrovascular accidents (12, 13) and Alzheimer's disease (14) and can result in LPL deficiency and consequent hyperlipidemia (15). LPL expression is a prognostic marker in B cell chronic lymphocytic leukemia (16) and has been linked to solid tumor cell proliferation (17). As LPL plays a critical role in several diseases, it is a therapeutic target for both inhibition (18) and induction (19). The LPL enzyme activity can be inhibited by Recombinant Mouse ANGPTL3.

Item	Value
References	Wang, H. and R. H. Eckel (2009) Am. J. Physiol. Endocrinol. Metab. 297:E271. Olivecrona, T.G. et al. (1985) J. Biol. Chem. 260:6888. Dugi, K. A. (1995) J. Biol. Chem. 270:25396. Keiper, T. et al. (2001) J. Lipid Res. 42:1180. Camps, L. et al. (1991) J. Lipid Res. 32:1877. Savonen, R. et al. (2015) J. Lipid Res. 56:588. Ping-Ping, H. et al. (2018) Clin Chim Acta 480:126. Takahashi, M. et al. (2013) J. Lipid Res. 54:1124. Qui, G. et al. (2007) J. Lipid Res. 48:385. Gagne, S. E. et al. (1999) Clin. Genet. 55:450. Jensen, M. K. et al. (2009) Am. Heart J. 157:384. Wang, C. et al. (2011) Thromb. Res. 128:e107. Zhang, W. S. et al. (2015) Int. J. Clin. Exp. Med. 8:9575. Ren, L. and X. Ren (2016) Neurosci. Lett. 619:73. Chan, L. Y. S. et al. (2002) Hum. Mutat. 20:232. Van Bockstaele, F. et al. (2007) Clin. Chem. 53:204. Kuemmerle, N.B. et al. (2011) Mol. Cancer Ther. 10:427. He, D. et al. (2016) J. Bioinform. Comput. Biol. 14:1650028. Takasu, S. et al. (2012) Biochem. Res. 2012:398697.
Entrez Gene IDs	4023 (Human); 16956 (Mouse); 24539 (Rat)
Alternate Names	EC 3.1.1, HDLCQ11, LIPD, LIPDEC 3.1.1.34, LPL, Lipoprotein Lipase

Citations for Recombinant Human Lipoprotein Lipase/LPL Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Lipoprotein Lipase/LPL Protein, CF include:

Species: Human
Sample Types: Plasma
Applications: Bioassay

R Risti et al. (2023-04-12), Combined action of albumin and heparin regulates lipoprotein lipase oligomerization, stability, and ligand interactions PLoS ONE, 2023-018(4):e0283358.
PMID: 37043509
Species: Human
Sample Types: Plasma
Applications: Bioassay

R Risti et al. (2023), Combined action of albumin and heparin regulates lipoprotein lipase oligomerization, stability, and ligand interactions PLoS ONE, 18(4):e0283358.
PMID: 37043509

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