Chinese Hamster Ovary cell line, CHO-derived human Lipoprotein Lipase/LPL protein Ala28-Gly475 with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
55-64 kDa, reducing conditions
Measured by its ability to hydrolyze 4-Nitrophenyl butyrate. The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.
Recombinant Human Lipoprotein Lipase/LPL Protein, CF Scientific Data Examples
Recombinant Human Lipoprotein Lipase/LPL Protein Bioactivity
Recombinant Human LPL activity can be inhibited by Recombinant Mouse ANGPTL3. Recombinant Mouse ANGPTL3 (Catalog # 9899-AN) dose dependently inhibits Recombinant Human LPL (Catalog # 9888-LL) activity with a ND50of 2-10 µg/mL.
Recombinant Human Lipoprotein Lipase/LPL Protein Enzyme Activity
Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is measured by its ability to hydrolyze 4-Nitrophenyl butyrate.
Recombinant Human Lipoprotein Lipase/LPL Protein SDS-PAGE
1 μg/lane of Recombinant Human Lipoprotein Lipase was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a band at 60 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CHAPS and Glycerol.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
Dilute rhLPL to 2 µg/mL in Assay Buffer.
Load 100 µL of Assay Buffer to all wells.
Add 50 μL of 2 µg/mL rhLPL to all wells.
Dilute Substrate to 8 mM in Substrate Buffer.
Add 50 μL of diluted Substrate to all wells. For Substrate Blank, load 150 μL of Assay Buffer followed by the addition of 50 μL of diluted Substrate.
Read in kinetic mode for 5 minutes at an absorbance of 400 nm.
Calculate specific activity:
Specific Activity (pmol/min/µg) =
Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)
amount of enzyme (µg)
*Adjusted for Substrate Blank. **Derived using calibration standard 4-Nitrophenol (4-NP) (Sigma, Catalog # 241326). Note: the output of many spectrophotometers is in mOD.
rhLPL: 0.1 μg
Substrate: 2 mM
Background: Lipoprotein Lipase/LPL
Lipoprotein Lipase (LPL) is a rate-limiting enzyme responsible for the hydrolysis of triglycerides (1). LPL forms a non-covalent active homodimeric molecule (2). Monomeric LPL contains an N-terminal domain with the catalytic triad responsible for lipolysis and a 22-amino acid loop that serves as a cover for the catalytic site (3) in addition to a C-terminal domain that contains the region required for dimerization (4) as well as the primary heparin-binding domain that is important for lipoprotein binding. LPL is expressed in many tissues (5, 6) where it is synthesized in the ER of parenchymal cells and secreted to capillaries. LPL is highly controlled by regulatory factors such as apolipoproteins, angiopoietins, and hormones (7). LPL can be produced by macrophages and this expression is a critical event in the pathogenesis of atherosclerosis (8) in addition to contributing to the macrophage inflammatory response (9). Variants of LPL have been associated with altered risk of several diseases including coronary heart disease (10, 11), cerebrovascular accidents (12, 13) and Alzheimer's disease (14) and can result in LPL deficiency and consequent hyperlipidemia (15). LPL expression is a prognostic marker in B cell chronic lymphocytic leukemia (16) and has been linked to solid tumor cell proliferation (17). As LPL plays a critical role in several diseases, it is a therapeutic target for both inhibition (18) and induction (19). The LPL enzyme activity can be inhibited by Recombinant Mouse ANGPTL3.
Wang, H. and R. H. Eckel (2009) Am. J. Physiol. Endocrinol. Metab. 297:E271.
Olivecrona, T.G. et al. (1985) J. Biol. Chem. 260:6888.
Dugi, K. A. (1995) J. Biol. Chem. 270:25396.
Keiper, T. et al. (2001) J. Lipid Res. 42:1180.
Camps, L. et al. (1991) J. Lipid Res. 32:1877.
Savonen, R. et al. (2015) J. Lipid Res. 56:588.
Ping-Ping, H. et al. (2018) Clin Chim Acta 480:126.
Takahashi, M. et al. (2013) J. Lipid Res. 54:1124.
Qui, G. et al. (2007) J. Lipid Res. 48:385.
Gagne, S. E. et al. (1999) Clin. Genet. 55:450.
Jensen, M. K. et al. (2009) Am. Heart J. 157:384.
Wang, C. et al. (2011) Thromb. Res. 128:e107.
Zhang, W. S. et al. (2015) Int. J. Clin. Exp. Med. 8:9575.
Ren, L. and X. Ren (2016) Neurosci. Lett. 619:73.
Chan, L. Y. S. et al. (2002) Hum. Mutat. 20:232.
Van Bockstaele, F. et al. (2007) Clin. Chem. 53:204.
Kuemmerle, N.B. et al. (2011) Mol. Cancer Ther. 10:427.
He, D. et al. (2016) J. Bioinform. Comput. Biol. 14:1650028.
Takasu, S. et al. (2012) Biochem. Res. 2012:398697.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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