Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Name: Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF
Brand: R&D Systems
SKU: AVI10544
Price: 527 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI10544

Product Datasheet / COA / SDS

Biotinylated

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Key Product Details

Source:	CHO
Accession #:	Q9BYF1.2
Structure / Form:	Biotinylated via Avi-tag
Applications:	Bioactivity, Enzyme Activity

View Full Technical Details for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Product Specifications for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Source

Chinese Hamster Ovary cell line, CHO-derived human ACE-2 protein

Human ACE-2 (Gln18-Ser740) Accession # Q9BYF1.2	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Protein identity confirmed by mass spectrometry.

Predicted Molecular Mass

112.0 kDa

SDS-PAGE

118-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) binds with an ED₅₀ of 0.6-4.8. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >500 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

	Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO) Binding Activity When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) binds with an ED₅₀ of 0.6-4.8 ng/mL.
	Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO) Enzyme Activity Biotinylated Recombinant Human ACE‑2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).
	Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO) SDS-PAGE 2 μg/lane of Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (Catalog # AVI10544) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~124 kDa under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10544

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl₂ and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

Item	Value
References	Kuba, K. et al. (2010) Pharmacol. Ther. 128:119. Yan, R. et al. (2020) Science 367:1444. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238. Kuba, K. et al. (2005) Nature Med. 11:875. Hoffmann, M. et al. (2020) Cell.181:1. Wrapp, D. et al. (2020) Science 367:1260. Imai, Y. et al. (2005) Nature 436:112. Huang, L. et al. (2003) J. Biol. Chem. 278:15532. Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350. Jia, H. et al. (2016) Shock. 46:239. Cole-Jeffrey, C.T. et al. (2015) J. Cardiovasc. Pharmacol. 66:540. Towler, P. et al. (2004) J. Biol. Chem. 279:17996. Crackower, M.A. et al. (2002) Nature 417:822.
Long Name	Angiotensin I Converting Enzyme 2
Entrez Gene IDs	59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names	ACE-2, ACE-related carboxypeptidase, ACE2, ACEH, ACEHangiotensin I converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2

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Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Scientific Data Examples for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ACE-2

Citations for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Product Documents for Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (CHO), CF

Product Datasheets

COA

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