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Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

R&D Systems, Inc. a Bio-Techne Brand

Substrate for ACE-2 and Caspase-1
Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
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ES007
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Key Product Details

Source: N/A
Applications: Bioactivity

Citations for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate (18)

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Customer Reviews for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate (2)

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  • Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 12/04/2020
    Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate Enzymatic activity in vitro ES007
  • Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
    Name: Shrinidh Joshi
    Verified Customer | Posted 08/04/2016
    ACE-2 activity was determined following incubation with intramolecularly quenched synthetic ACE-2 specific substrate 7-Mca-YVADAPK (Dnp) (R&D systems). In the case of cell lysates, 10 µg of total protein was assayed for activity in a buffer with the following composition: 50 mM MES (4-morpholineethanesulphonic acid), 300 mM NaCl, 10 µm ZnCl2 and 0.01% Triton X-100, pH 6.5. Reaction was initiated by the addition of 5×10−5 M substrate. Where applicable, recombinant enzymes were used at a concentration of 0.01 µg per reaction. The fluorescence measurements were performed in the black microtiter plates (Costar) in a total volume of 100 µl. The plates were read using a fluorescence plate reader SpectraMax M5 (Molecular Devices) at an excitation wavelength 320 nm and emission wavelength 405 nm Fluorescence resulting from the substrate hydrolysis increased with time, and achieved maximum by one h with recombinant enzyme.
    Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate ES007

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Product Documents for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

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