Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source: | N/A |
Applications: | Bioactivity |
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Citations for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate (18)
Citations are publications that use Bio-Techne products. Selected citations for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate include:
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Species: Human
Sample Types: Peptides
Applications: BioassayWG Liang et al. (2022), Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition Nature Communications, 13(1):1833.
PMID: 35383169 -
Species: Human
Sample Types: Recombinant Protein
Applications: BioassayJA Torchia et al. (2022), Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivo Science Advances, 8(49):eabq6527.
PMID: 36475798 -
Species: Human
Sample Types: Recombinant Protein
Applications: BioassaySP Shukla et al. (2021), "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry International Journal of Molecular Sciences, 22(16).
PMID: 34445669 -
Species: Human
Sample Types: Cell Lysates
Applications: Hydrolysis AssayGM Castiglion et al. (2021), Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2 PloS Biology, 19(12):e3001510.
PMID: 34932561 -
Applications: Enzyme AssayJ Lu et al. (2020), High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity bioRxiv, 0(0).
PMID: 32637947 -
Species: Human
Sample Types: Protein
Applications: BioassayKY Huang et al. (2020), Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection EMBO Mol Med, 0(0):e12828.
PMID: 33159417 -
Species: Mouse
Sample Types: Cells
Applications: enzyme activityK Hao et al. (2019), LncRNA-Safe contributes to cardiac fibrosis through Safe-Sfrp2-HuR complex in mouse myocardial infarction Theranostics, 9(24):7282-7297.
PMID: 31695768 -
Species: Human
Sample Types: Tissue Homogenates
E Schrom et al. (2017), Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 7(0):350-365.
PMID: 28624211 -
Species: Human
Sample Types: Plasma
Applications: Enzyme AssayPutko B et al. (2014), Circulating levels of tumor necrosis factor-alpha receptor 2 are increased in heart failure with preserved ejection fraction relative to heart failure with reduced ejection fraction: evidence for a divergence in pathophysiology. PLoS ONE, 9(6):e99495.
PMID: 24923671 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Enzyme AssayQiu Y et al. (2014), Angiotensin-converting enzyme 2 (ACE2) activator diminazene aceturate ameliorates endotoxin-induced uveitis in mice. Invest Ophthalmol Vis Sci, 55(6):3809-18.
PMID: 24854854 -
Species: Mouse
Sample Types: Tissue Homogenates
Riquelme C et al. (2014), ACE2 is augmented in dystrophic skeletal muscle and plays a role in decreasing associated fibrosis. PLoS ONE, 9(4):e93449.
PMID: 24695436 -
Species: Human
Sample Types: Cell Lysates
Applications: BioassayYan et al. (2013), Dicer expression exhibits a tissue-specific diurnal pattern that is lost during aging and in diabetes. PLoS ONE, 8(11):e80029.
PMID: 24244599 -
Species: Rat
Sample Types: Whole Tissue
Applications: BioassayBhatia et al. (2013), Sex differences in angiotensin-converting enzyme modulation of Ang (1-7) levels in normotensive WKY rats. Am J Hypertens, 26(5):591-8.
PMID: 23547034 -
Species: Human
Applications: BioassayTsuchiya S et al. (2010), Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp). J. Bone Miner. Res., 26(0):220.
PMID: 20687161 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Enzyme AssayPark S et al. (2010), Major role for ACE-independent intrarenal ANG II formation in type II diabetes. Am. J. Physiol. Renal Physiol., 298(1):F37-48.
PMID: 19846569 -
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay SubstrateBergin DA et al. (2008), Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway. J. Biol. Chem., 283(46):31736-44.
PMID: 18772136 -
Species: Human
Sample Types: Cell Culture Supernates
Lovren F et al. (2008), Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis. Am. J. Physiol. Heart Circ. Physiol., 295(4):H1377-84.
PMID: 18660448 -
Species: Human
Sample Types: Peptide
Applications: BioassayLu J et al. (0), High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. J Biol Che295(52):18579-18588.
PMID: 33122196
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Customer Reviews for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate (2)
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Application: Enzymatic activity in vitroVerified Customer | Posted 12/04/2020
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Verified Customer | Posted 08/04/2016ACE-2 activity was determined following incubation with intramolecularly quenched synthetic ACE-2 specific substrate 7-Mca-YVADAPK (Dnp) (R&D systems). In the case of cell lysates, 10 µg of total protein was assayed for activity in a buffer with the following composition: 50 mM MES (4-morpholineethanesulphonic acid), 300 mM NaCl, 10 µm ZnCl2 and 0.01% Triton X-100, pH 6.5. Reaction was initiated by the addition of 5×10−5 M substrate. Where applicable, recombinant enzymes were used at a concentration of 0.01 µg per reaction. The fluorescence measurements were performed in the black microtiter plates (Costar) in a total volume of 100 µl. The plates were read using a fluorescence plate reader SpectraMax M5 (Molecular Devices) at an excitation wavelength 320 nm and emission wavelength 405 nm Fluorescence resulting from the substrate hydrolysis increased with time, and achieved maximum by one h with recombinant enzyme.
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