Media for the ex vivo culture of B lymphocytes under xeno-free conditions.
- Robust B cell expansion under xeno-free conditions
- Facilitates the isolation and expansion of B cells from peripheral blood
- Compatible for culturing B cells from multiple species
- Supports target antigen-specific B cell clonogenicity
- Supports culture of mouse B cell hybridoma cell lines for antibody production
Why Use ExCellerate B Cell Media?
B lymphocytes (B cells) play an essential role in the humoral component of the adaptive immune response. Following exposure to a pathogen or foreign material, B cells produce and secrete antibodies that circulate through the body and bind to specific pathogens, marking them for destruction. B cells also contribute to cellular immunity by maintaining immune homeostasis, including the regulation of T lymphocyte activation and expansion. Diseases or abnormalities that affect B cells have been shown to result in immunodeficiencies, autoimmunity, and cancer. Continued progress in basic and clinical B cell research is crucial for further understanding human health and disease.
ExCellerate B Cell Media is formulated and optimized for the ex vivo culture of B lymphocytes under xeno-free conditions. Unlike traditional serum-containing media, ExCellerate B Cell Media provides a stable and optimized culture environment, void of non-human animal-derived products, that facilitates the isolation and expansion of B cells from peripheral blood. This media supports target antigen-specific B cell clonogenicity as well as robust culture of mouse B cell hybridoma cell lines for antibody production.
Versatility of ExCellerate B Cell Media.
ExCellerate B Cell Media can be used with cytokine/growth factor supplements for the ex vivo culture of B cells. This versatile medium is compatible for culturing B cells from multiple species (i.e., human, mouse, rabbit, and canine). Cytokine/growth factor combination and concentrations should be used with this media should be optimized by application or experimental protocol.
- 500 mL of ExCellerate B Cell Media, Xeno-Free
The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long maintenance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continuously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage depletion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.