CellXVivo Human B Cell Expansion Kit
R&D Systems, part of Bio-Techne | Catalog # CDK005
For the proliferative expansion of human B cell populations.
Key Product Details
Species
Human
Product Summary for CellXVivo Human B Cell Expansion Kit
Kit Summary
For the proliferative expansion of B cell populations.
Key Benefits
- Yields a 3-5 fold expanded population of B cells
- Provides optimized reagents needed to induce B cell proliferation
- Utilizes validated and straightforward procedures
- Does not require specialized instrumentation
Why Expand B Cells Ex Vivo?
B cells are a subset of lymphocytes that express immunoglobulins on their cell surface. The B cell population is a vital component of the humoral immune response. B cell activation leads to antigen uptake and presentation, affinity maturation and class switching of the immunoglobulins, and antibody secretion. B cells represent a small portion (~5%) of the peripheral lymphocyte population making it difficult to obtain sufficient B cell quantities for many research applications. The ability to expand B cell populations ex vivo is valuable to researchers who require B cells for downstream applications including high throughput B cell assays and ex vivo differentiation of T cells.
Kit Contents
This kit contains the following optimized reagents for the expansion of human B cell populations.
- B Cell Expander Reagents
- Reconstitution Buffers
- Provides enough reagents for the 3-5 fold expansion of a starting population of 107 human B cells
Stability and Storage
Store the unopened kit at 2-8 °C. Do not use past the kit expiration date. Opened or reconstituted B Cell Expander 1, B Cell Expander 2, and B Cell Expander 3 should be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months.* Opened Reconstitution Buffer 1 and Reconstitution Buffer 2 should be stored at 2-8 °C under sterile conditions for up to 12 months.*
*Provided this is within the expiration date of the kit.
Scientific Data Images for CellXVivo Human B Cell Expansion Kit
Light Microscopy of B Cells.
B cells were imaged at 20X magnification following isolation from PMBCs and (B) after 5 days in culture using reagents included in the Human B Cell Expansion Kit.
Detection of CD20 in Human B Cells.
Human B cells were expanded for 5 days using reagents included in the Human B Cell Expansion Kit. The cells were labeled with a PE-conjugated Mouse Anti-Human CD20 Monoclonal Antibody (R&D Systems, Catalog # FAB4225P; filled histogram) or a PE-conjugated Mouse IgG1 Isotype control (R&D Systems, Catalog # IC002P; open histogram).
Human B Cell Expansion.
Human B cells were isolated from PMBCs and cultured for 5 days using reagents included in the Human B Cell Expansion Kit. B cell expansion was measured with Resazurin (R&D Systems, Catalog # AR002).Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: B Cell Expansion Kits
B cells are a subset of lymphocytes that express immunoglobulins on their cell surface. The B cell population is a vital component of the humoral immune response. B cell activation leads to antigen uptake and presentation, affinity maturation and class switching of the immunoglobulins, and antibody secretion. B cells represent a small portion (~5%) of the peripheral lymphocyte population making it difficult to obtain sufficient B cell quantities for many research applications. The ability to expand B cell populations ex vivo is valuable to researchers who require B cells for downstream applications including high throughput B cell assays and ex vivo differentiation of T cells.
Additional B Cell Expansion Kits Products
Product Documents for CellXVivo Human B Cell Expansion Kit
Product Specific Notices for CellXVivo Human B Cell Expansion Kit
For research use only
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