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CellXVivo Human B Cell Expansion Kit

For the proliferative expansion of human B cell populations.
Light Microscopy of B Cells.
(4)
Detection of CD20 in Human B Cells.
Human B Cell Expansion. 
CellXVivo Human B Cell Differentiation Kit
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CDK005

Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, human B cell populations can be expanded using the following procedure:

  • Isolate human B cells from PBMC preparation
  • Culture B cells in B Cell Expander-supplemented media
  • Measure B cell expansion using Resazurin
 
Reagents Provided

Reagents Supplied in the CellXVivo™ Human B Cell Expansion Kit (Catalog # CDK005):

  • B Cell Expander 1
  • B Cell Expander 2
  • B Cell Expander 3
  • Reconstitution Buffer 1
  • Reconstitution Buffer 2

 

Other Supplies Required

Reagents

  • MagCellect™ Human B Cell Isolation Kit (R&D Systems, Catalog # MAGH103, or equivalent).
  • Resazurin (R&D Systems, Catalog # AR002, or equivalent).
  • Ficoll-Hypaque™
  • RPMI 1640
  • L-Glutamine
  • Penicillin
  • Streptomycin
  • Fetal Bovine Serum (FBS)
  • beta-Mercaptoethanol

Equipment

  • Tissue culture flasks and/or plates
  • Inverted microscope
  • Hemocytometer
  • 37 °C and 5% CO2 humidified cell culture incubator
  • Benchtop centrifuge
  • Pipettes and pipette tips

 

Procedure Overview

R&D Systems Protocol for Human B Cell expansion

Isolate PBMCs from human blood.

Isolate PBMCs from human blood

Isolate human B cells from a PBMC preparation (e.g., using magnetic cell selection).

Isolate human B Cells from PBMCs

Perform a cell count.

Perform a cell count

Culture 2 x 105 cells/mL in media supplemented with reconstituted B Cell Expanders 1-3.

Incubate the cells for 5 days.

Measure B cell expansion using Resazurin.

Culture cells in media

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    Verified Customer | Posted 04/04/2018

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FAQs

  • Can the CellXVivo Human B Cell Expansion Kit, catalog # CDK005, be used for expansion of memory B cells?

    Based on the components used in the CDK005 kit, the kit is expected to expand total B cells and is not specialized for memory B cells.

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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