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Recombinant Mouse IL-4 Protein

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Mouse IL-4 Protein Bioactivity
(2)
Recombinant Mouse IL-4 Protein SDS-PAGE
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With Carrier
404-ML-025
404-ML-100
404-ML-050
404-ML-01M
404-ML-010

Catalog #
Availability
Size / Price
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Carrier Free
404-ML-01M/CF
404-ML-050/CF
404-ML-100/CF
404-ML-010/CF
404-ML-025/CF
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Key Product Details

Source: E. coli
Accession #: P07750
Applications: Bioactivity

Citations for Recombinant Mouse IL-4 Protein (399)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-4 Protein include:

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Customer Reviews for Recombinant Mouse IL-4 Protein (16)

4.9 out of 5
16 customer ratings
5 stars
88%
4 stars
13%
3 stars
0%
2 stars
0%
1 star
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  • Recombinant Mouse IL-4 Protein
    Name: Katie Myers
    Application: Immunoassay Standard
    Verified Customer | Posted 11/14/2022
    Used as a standard in our mouse ELISA.
    Recombinant Mouse IL-4 Protein Immunoassay Standard 404-ML
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 09/29/2021
  • Recombinant Mouse IL-4 Protein
    Name: Marina Theodorou
    Application: CellProlif
    Verified Customer | Posted 05/20/2021
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/13/2021
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/27/2021
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 01/05/2021
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 01/05/2021
  • Recombinant Mouse IL-4 Protein
    Name: Gagandeep Kaur
    Application: CellProlif
    Verified Customer | Posted 11/09/2020
  • Recombinant Mouse IL-4 Protein
    Name: Yu Dang
    Application: In vitro bioactivity in cell culture
    Reason for Rating: IL-4 was used to differentiate THP-1 into M2 phenotype macrophages. We used immunofluorescence staining to show that after treatment the cells are CD206 positive, which means the treatment is successful in the phenotype change of THP-1 into M2 macrophage.
    Verified Customer | Posted 10/23/2020
    concentration :5ng/mlTime: 24 h
    Recombinant Mouse IL-4 Protein In vitro bioactivity in cell culture 404-ML
  • Recombinant Mouse IL-4 Protein
    Name: Supriya Sen
    Application: Immune-cell polarization.
    Verified Customer | Posted 10/06/2020
    Recombinant Mouse IL-4 Protein Other 404-ML
  • Recombinant Mouse IL-4 Protein
    Name: Yuqing Feng
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/16/2019
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 07/13/2018
  • Recombinant Mouse IL-4 Protein
    Name: Danlee Enzler
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/02/2018
  • Recombinant Mouse IL-4 Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: MAB404 binds rhuIL-13Ra2
    Verified Customer | Posted 02/07/2018
    Recombinant Mouse IL-4 Protein Binding assay/Protein-protein interaction 404-ML
  • Recombinant Mouse IL-4 Protein
    Name: Andrew Lechner
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/21/2016
    We used recombinant mouse IL-4 to polarize bonemarrow-derived macrophages to an "M2" phenotype in vitro. We first generated macrophages from whole bone marrow using MCSF-rich media for 7 days. Then we added IL-4 at 20 ng/ml for 48 hours. Using IL-4R wild-type or knock-out bone marrow, we demonstrated using flow cytometry for an M2 reporter (Arginase1-YFP) that the IL-4 acts through the IL-4 receptor to polarize bone marrow derived macrophages towards the M2 phenotype.
    Recombinant Mouse IL-4 Protein In vitro bioactivity in cell culture 404-ML
  • Recombinant Mouse IL-4 Protein
    Name: Tima Thomas
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016
    Recombinant Mouse IL-4 Protein In vitro bioactivity in cell culture 404-ML

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FAQs for Recombinant Mouse IL-4 Protein

  • Has recombinant mouse IL-4, Catalog # 404-ML, been tested for activity on human cells?

    We have not evaluated the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) with human cells. However, we recommend reviewing our list of publications under the Citations tab on the product web page to find reported use of this product with human cells. We do offer a Recombinant Human IL-4 Protein (Catalog # 204-IL), which is suitable for use with human cells.

  • What concentration of recombinant mouse IL-4, Catalog # 404-ML, should I use for my cell culture experiment?

    We evaluate the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.5-1.5 ng/mL.  This ED50 range can provide a starting point for your experiment, but we would recommend end-users perform a dose-response to determine the best concentration for their assay system.  We also recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.  

Product Documents for Recombinant Mouse IL-4 Protein

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.