Mouse CD40/TNFRSF5 Antibody

Name: Mouse CD40/TNFRSF5 Antibody
Brand: R&D Systems
SKU: MAB440
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB440

Citations (6)

(1)

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Species Reactivity:	Mouse
Immunogen:	COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse CD40/TNFRSF5 Extracellular domain
Applications:	CyTOF-ready, Flow Cytometry, Functional Assay, Immunoprecipitation
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 1C10
Specificity:	Specifically binds mouse CD40/TNFRSF5 expressed on B cells.

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Applications for Mouse CD40/TNFRSF5 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Mouse B220⁺ splenocytes
Immunoprecipitation	Heath, A.W. et al. (1994) Eur. J. Immunol. 24:1828.
Agonist Activity	Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. The ED₅₀ for this effect is typically 0.03-0.18 μg/mL (1).
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD40/TNFRSF5

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).

Item	Value
References	Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569. Armitage, R.J. et al. (1993) J. Immunol. 150:3671. Callard, R.E. et al. (1993) Immunol. Today 14:559. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487. Pullen, S.S. et al. (1999) Biochemistry 38:10168. Faris, M. et al. (1994) J. Exp. Med. 179:1923. Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.
Entrez Gene IDs	958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)
Alternate Names	B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, TNFRSF5, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), nerve growth factor receptor-related B-lymphocyte activation molecule, p50, tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

Citations for Mouse CD40/TNFRSF5 Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD40/TNFRSF5 Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

A Ortega-Mol et al. (2021), Inhibition of Rag GTPase signaling in mice suppresses B cell responses and lymphomagenesis with minimal detrimental trade-offs Cell Reports, 36(2):109372.
PMID: 34260908
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

ST Tivadar et al. (2019), Monoclonal Antibody Targeting Sialyl-di-Lewisa - Containing Internalizing and non-Internalizing Glycoproteins with Cancer Immunotherapy Development Potential Mol. Cancer Ther., 0(0).
PMID: 31871270
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Pierson E et al. (2014), B cells promote induction of experimental autoimmune encephalomyelitis by facilitating reactivation of T cells in the central nervous system. J Immunol, 192(3):929-39.
PMID: 24367024
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lechouane F et al. (2013), B-cell receptor signal strength influences terminal differentiation. Eur J Immunol, 43(3):619-28.
PMID: 23280426
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Liu J et al. (2007), Suppressive effect of reactive oxygen species on CD40-induced B cell activation. FEBS Lett., 581(26):5043-9.
PMID: 17919601
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

de la Fuente MA et al. (2006), 3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation. Mol. Cell. Biol., 26(14):5214-25.
PMID: 16809760

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Customer Reviews for Mouse CD40/TNFRSF5 Antibody (1)

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Name: Anonymous

Application: Flow

Sample Tested: Whole Cells

Species: Mouse

Verified Customer | Posted 07/20/2017

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Mouse CD40/TNFRSF5 Antibody

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