Mouse S100A8 Antibody

Name: Mouse S100A8 Antibody
Brand: R&D Systems
SKU: AF3059

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF3059

Citations (20)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse S100A8 Pro2-Glu89 Accession # P27005
Applications:	Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.

View Full Technical Details for Mouse S100A8 Antibody

Complete Your Research

Applications for Mouse S100A8 Antibody

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse S100A8 Antibody

	Western Blot Detection of Mouse S100A8 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A8 at approximately 10-11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry S100A8 in Mouse Splenocytes. S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunocytochemistry S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Simple Western Detection of Mouse S100A8 by Simple Western^TM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 9 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: S100A8

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha -helix, a Ca⁺⁺-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca⁺⁺, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively.

Item	Value
Long Name	S100 Calcium Binding Protein A8
Entrez Gene IDs	6279 (Human); 20201 (Mouse)
Alternate Names	60B8AG, CAGACP-10, CFAG, CFAGL1Ag, CGLA, Calgranulin A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MA387, MIF, MRP-8, MRP8, MRP8S100 calcium binding protein A8 (calgranulin A), Migration inhibitory factor-related protein 8, NIF, P8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, Urinary stone protein band A, calgranulin-A, protein S100-A8

Citations for Mouse S100A8 Antibody (20)

Showing 1 - 10 of 20 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse S100A8 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

G Theocharid et al. (2022), Single cell transcriptomic landscape of diabetic foot ulcers Nature Communications, 13(1):181.
PMID: 35013299
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

C Conrad et al. (2022), ADAM8 signaling drives neutrophil migration and ARDS severity JCI Insight, 7(3).
PMID: 35132956
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

YT Ho et al. (2021), Longitudinal single cell transcriptomics reveals a role for Serpina3n-mediated resolution of inflammation in a mouse colitis model Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 33862275
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

MJ Kim et al. (2020), Reduced Fecal Calprotectin and Inflammation in a Murine Model of Atopic Dermatitis Following Probiotic Treatment Int J Mol Sci, 21(11).
PMID: 32486523
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

S Maekawa et al. (2019), RNA sequencing for ligature induced periodontitis in mice revealed important role of S100A8 and S100A9 for periodontal destruction Sci Rep, 9(1):14663.
PMID: 31605018
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Dufour et al. (2018), C-terminal truncation of IFN-? inhibits proinflammatory macrophage responses and is deficient in autoimmune disease Nat Commun, 9(1):2416.
PMID: 29925830
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

YE Kim et al. (2018), Hypoxia-inducible factor-1 (HIF-1) activation in myeloid cells accelerates DSS-induced colitis progression in mice Dis Model Mech, 0(0).
PMID: 29967068
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

(2016), Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 0(0).
PMID: 27382022
(2016), Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content Sci Rep, 6(0):37871.
PMID: 27917875
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Thomsen M et al. (2015), Loss of JUNB/AP-1 promotes invasive prostate cancer. Cell Death Differ, 22(4):574-82.
PMID: 25526087
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Behnsen J et al. (2014), The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity, 40(2):262-73.
PMID: 24508234
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Baluk P et al. (2014), Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation. Am J Pathol, 184(5):1577-92.
PMID: 24631179
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Kim H et al. (2014), S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model. PLoS ONE, 9(2):e88924.
PMID: 24586443
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Yajima-Himuro et al. (2014), The junctional epithelium originates from the odontogenic epithelium of an erupted tooth. Sci Rep, 4(0):4867.
PMID: 24785116
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Baluk P et al. (2014), Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways. Am J Pathol, 184(6):1877-89.
PMID: 24726646
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Yano J et al. (2014), Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis. , 0(0).
PMID: 24478092
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Sade-Feldman M et al. (2013), Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation. Immunity, 38(3):541-54.
PMID: 23477736
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Gonzalez-Lopez A et al. (2012), MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia. PLoS ONE, 7(6):e39940.
PMID: 22768176
Species: Mouse
Sample Types: Vaginal Lavage Fluid
Applications: ELISA Development

Yano et al. (2012), The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway. PLoS ONE, 7(9):e46311.
PMID: 23050010
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Cuervo H et al. (2011), Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection. J. Immunol., 187(5):2656-65.
PMID: 21804013

There are no citations that match your criteria.

Showing 1 - 10 of 20 citations Showing All

Product Documents for Mouse S100A8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS