Mouse S100A8 Biotinylated Antibody

Name: Mouse S100A8 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3059
Price: 483 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF3059

Citations (2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse S100A8 Pro2-Glu89 Accession # P27005
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse S100A8 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, recombinant human (rh) S100B, and rhS100P is observed.

View Full Technical Details for Mouse S100A8 Biotinylated Antibody

Applications for Mouse S100A8 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse S100A8
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse S100A8 Biotinylated Antibody

	Immunocytochemistry S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: S100A8

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha -helix, a Ca⁺⁺-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca⁺⁺, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively.

Item	Value
Long Name	S100 Calcium Binding Protein A8
Entrez Gene IDs	6279 (Human); 20201 (Mouse)
Alternate Names	60B8AG, CAGACP-10, CFAG, CFAGL1Ag, CGLA, Calgranulin A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MA387, MIF, MRP-8, MRP8, MRP8S100 calcium binding protein A8 (calgranulin A), Migration inhibitory factor-related protein 8, NIF, P8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, Urinary stone protein band A, calgranulin-A, protein S100-A8

Citations for Mouse S100A8 Biotinylated Antibody (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse S100A8 Biotinylated Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

E Källberg et al. (2018), Induction of S100A9 homodimer formation inï¿½vivo Biochem. Biophys. Res. Commun., 0(0).
PMID: 29679568
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development

Reyes L et al. (2009), Complicated urinary tract infection is associated with uroepithelial expression of proinflammatory protein S100A8. Infect. Immun., 77(10):4265-74.
PMID: 19667050

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Product Documents for Mouse S100A8 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Mouse S100A8 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Mouse S100A8 Biotinylated Antibody

Scientific Data Examples for Mouse S100A8 Biotinylated Antibody

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: S100A8

Citations for Mouse S100A8 Biotinylated Antibody (2)

Product Documents for Mouse S100A8 Biotinylated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected