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Mouse IL-1 beta /IL-1F2 Antibody

Detection of Human and Mouse IL-1 beta /IL-1F2 antibody by Western Blot.
(5)
IL-1 beta /IL-1F2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).
IL-1 beta /IL-1F2 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL-1 beta /IL-1F2 Antibody.
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AF-401-NA
AF-401-SP

Key Product Details

Species Reactivity: Mouse
Immunogen: E. coli-derived recombinant mouse IL-1 beta /IL-1F2
Val118-Ser269
Accession # NP_032387
Applications: Block/Neutralize, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label: Unconjugated
Source: Polyclonal Goat IgG
Specificity: Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots.

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Citations (175)

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Customer Reviews (16)

3.9 out of 5
16 customer ratings
5 stars
19%
4 stars
63%
3 stars
13%
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1 star
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  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: mouse oral tissue
    Species: Mouse
    Verified Customer | Posted 11/23/2020
    Mouse IL-1 beta /IL-1F2 Antibody Immunohistochemistry AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 11/19/2020
    Human-derived THP-1 cells either left unstimulated or treated with LPS+ATP for 3 hours. Intracellular detection.
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Brain (caudate putamen)
    Species: Mouse
    Verified Customer | Posted 05/23/2019
    I am somewhat disappointed. My lab published 4 years ago an article using this Ab. I boight it last september.There is no way I can get the bands ourselves and other authors have published: the proIL1-beta and the mature form, at around 35 and 17kDa. Attached is a representative blot of the many I have run.I have tried homemade 12% gels, BioRad 12 and 4-20% precast gels. I have tried both types of membranes (PVDF and Nitro 0.2), blocking with milk and BSA, different transfer times (35min 120V, 45min 100V, 1h 100V, 1.5h 100V), different Ab dilutions. And I still get nothing that comes to close to the pro-IL-1beta, and of course, the mature form is nowhere to be found.I do not wish to buy another brand, I believe this to be a good Ab, but since it is polyclonal, it might give different results depending on the lot.
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: IC-21 mouse macrophage cell line
    Species: Mouse
    Verified Customer | Posted 01/28/2019
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: IC-21 mouse macrophage cell line
    Species: Mouse
    Verified Customer | Posted 01/28/2019
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 08/08/2018
    10.1093/cercor/bhy164
    Mouse IL-1 beta /IL-1F2 Antibody Immunohistochemistry AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line
    Species: Mouse
    Verified Customer | Posted 07/17/2018
    Mouse IL-1 beta /IL-1F2 Antibody Simple Western AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Cell Lysates,Macrophages Cell Supernatant
    Species: Mouse,Mouse macrophages
    Verified Customer | Posted 07/17/2018
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 07/17/2018
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/17/2018
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: IC-21 mouse macrophage cell line
    Species: Mouse
    Verified Customer | Posted 03/22/2018
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: bone marrow derived macrophage
    Species: Mouse
    Verified Customer | Posted 10/31/2017
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: L-929 mouse fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 09/08/2017
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: B/N
    Sample Tested: Adult splenocytes
    Species: Mouse
    Verified Customer | Posted 04/26/2017
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Bone marrow cells,Cell culture supernatant,Serum-free Cell Culture Supernates
    Species: Mouse
    Verified Customer | Posted 04/25/2017
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA
  • Mouse IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: bone marrow derived macrophages
    Species: Mouse
    Verified Customer | Posted 02/14/2017
    Mouse IL-1 beta /IL-1F2 Antibody Western Blot AF-401-NA

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FAQs

  • Does catalog # AF-401-NA detect the pro or mature form of IL-1 beta in western blot?

    This would depend on the sample being used. The pro-form of the protein also contains the sequence for the mature form.  When cell lysates are used as the sample, the pro-form is mainly detected.  When cell culture supernatants containing mainly the mature form of the protein is used, only the mature form is detected.  This is exemplified in the following paper:J Immunol.(2015) 195, 1233.  http://www.jimmunol.org/content/195/3/1233/tab-figures-data

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