Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

Name: Mouse CX3CL1/Fractalkine Chemokine Domain Antibody
Brand: R&D Systems
SKU: AF472
Price: 182 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF472

Citations (10)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse CX3CL1/Fractalkine Leu22-Lys105 Accession # O35188
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Fractalkine in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human Fractalkine and recombinant rat Fractalkine is observed.

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Applications for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CX3CL1/Fractalkine Full Length

Item	Value
Neutralization	Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CX3CL1/Fractalkine aa 25‑105.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

	Neutralization Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND₅₀ is typically 0.3-1.5 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CX3CL1/Fractalkine

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Item	Value
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody (10)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Sun et al. (2022), Diet-Induced Obesity Promotes Liver Metastasis of Pancreatic Ductal Adenocarcinoma via CX3CL1/CX3CR1 Axis Journal of Immunology Research, 2022(0):5665964.
PMID: 35478937
M Bolós et al. (2017), Absence of CX3CR1 impairs the internalization of Tau by microglia Mol Neurodegener, 12(1):59.
PMID: 28810892
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Bryan Simons (2016), PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen J. Immunol, 0(0).
PMID: 27895167
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Yoh Goto (2016), CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow Stem Cells, 0(0).
PMID: 27339271
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Morimura S et al. (2013), Interaction between CX3CL1 and CX3CR1 regulates vasculitis induced by immune complex deposition. Am J Pathol, 182(5):1640-7.
PMID: 23470165
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Eberlein J et al. (2010), Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 120(3):907-23.
PMID: 20197626
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Ishida Y et al. (2008), Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis. J. Immunol., 181(6):4208-18.
PMID: 18768878
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Ishida Y et al. (2008), Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function. J. Immunol., 180(1):569-79.
PMID: 18097059
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Ren T et al. (2007), Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice. Biochem. Biophys. Res. Commun., 364(4):978-84.
PMID: 17971299
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Ueha S et al. (2007), Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects. J. Leukoc. Biol., 81(1):176-85.
PMID: 17053165

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Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

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Applications for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

Scientific Data Examples for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody (10)

Product Documents for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

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