Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein

Name: Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein
Brand: R&D Systems
SKU: 571-MF
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 571-MF

Citations (3)

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Source:	E. coli
Accession #:	AAB71763
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein

Product Specifications for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein

Source	E. coli-derived mouse CX3CL1/Fractalkine protein Gln25-Lys105
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln25 predicted
Predicted Molecular Mass	9.3 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED₅₀for thiseffect is 0.004‑0.02 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

571-MF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

571-MF/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

Fractalkine, designated CX3CL1 and also known as neurotactin, is the only member of the CX3C, or delta, chemokine subfamily (1‑4). Unlike most other chemokines, CX3CL1 is a type I transmembrane (TM) adhesion protein (1). The mouse CX3CL1 cDNA encodes 395 amino acids (aa), including a signal sequence (aa 1‑24), a chemokine domain (aa 25‑100), a mucin stalk region (aa 101‑336), a transmembrane segment (aa 337‑357), and a cytoplasmic tail (aa 358‑395). The chemokine domain contains binding and chemotactic determinants, while the mucin stalk appears to function only as a spacer (4, 5). Mouse CX3CL1 shares 85% and 78% aa sequence identity with rat and human CX3CL1, respectively, within the chemokine domain, but lower sequence identity within other domains. CX3CL1 is up‑regulated by pro‑inflammatory stimuli, especially IFN‑ gamma and TNF‑ alpha, on cell types including macrophages, dendritic cells, endothelium, neurons, smooth muscle and epithelium lining the intestines and other tubules (1, 8, 9). The 40 kDa, 7‑TM non‑glycosylated G‑protein coupled CX3CL1 receptor, CX3CR1, is expressed by cytotoxic effector cells and cytokine producers, including type I helper and cytotoxic T cells, gamma δ T cells, CD16+ NK cells, monocytes and microglia (1, 2). The 95‑100 kDa TM CX3CL1 can be inducibly cleaved near the TM segment by ADAM10 or ADAM17 to generate a 60‑80 kDa soluble form (6, 7). TM CX3CL1 functions as an adhesion molecule, while both forms are chemoattractants for target cells expressing CX3CR1 (1, 2). During extravasation, membrane‑bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis (6). In coronary artery disease, soluble CX3CL1 and CD8+ T cell CX3CR1 are over-expressed and appear to contribute to pathogenesis (1, 10). In the brain, CX3CL1/CX3CR1 interaction protects against microglial neurotoxicity (11). CX3CL1 also contributes to wound healing by recruiting macrophages, and to bone resorption by recruiting and mediating adhesion of osteoclast precursors (12, 13).

Item	Value
References	Stievano, L. et al. (2004) Crit. Rev. Immunol. 24:205. Umehara, H. et al. (2004) Arterioscler. Thromb. Vasc. Biol. 24:34. Rossi, D.L. et al. (1998) Genomics 47:163. Mizoue, L.S. et al. (2001) J. Biol. Chem. 276:33906. Harrison, J.K. et al. (2001) J. Biol. Chem. 276:21632. Hundhausen, C. et al. (2007) J. Immunol. 178:8064. Tsou, C. et al. (2001) J. Biol. Chem. 276:44622. Tarozzo, G. et al. (2003) J. Neurosci. Res. 73:81. Lucas, A.D. et al. (2001) Am. J. Pathol. 158:855. Damas, J.K. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:2567. Cardona, A.E. et al. (2006) Nat. Neurosci. 9:917. Koizumi, K. et al. (2009) J. Immunol. 183:7825. Ishida, Y. et al. (2008) J. Immunol. 180:569.
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein include:

A Wang et al. (2018), IP3-Mediated Calcium Signaling Is Involved in the Mechanism of Fractalkine-Induced Hyperalgesia Response Med. Sci. Monit., 24(0):8804-8811.
PMID: 30517088
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Park et al. (2017), Fractalkine induces angiogenic potential in CX3CR1-expressing monocytes J. Leukoc. Biol., 0(0).
PMID: 28978662
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Gevrey JC et al. (2005), Syk is required for monocyte/macrophage chemotaxis to CX3CL1 (Fractalkine). J. Immunol., 175(6):3737-45.
PMID: 16148119

Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein

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Product Specifications for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein

Formulation, Preparation and Storage

Carrier Free

571-MF

Carrier

571-MF/CF

Carrier Free

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein (3)

Product Documents for Recombinant Mouse CX3CL1/Fractalkine (aa 25-105) Protein

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