Cathepsin X/Z/P was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1033) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Cathepsin X/Z/P Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Cathepsin X/Z/P (0.1 µg/mL, Catalog # 1033-CY) cleavage of the fluorogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH (10 µM, Catalog # ES005). The Neutralization Dose (ND₅₀) is 8 - 73 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References

Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
Nagler and Menard (1998) FEBS Lett. 434:135.
Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
Pungercar, et al. (2000) Pflugers Arch. 439:R119.
Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
Menard, et al. (2001) Biol. Chem. 382:839.
Therrien, et al. (2001) Biochemistry 40:2702.
Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
Guncar, et al. (2000) Structure Fold Des. 8:305.
Lustigman, et al. (1996) J. Biol. Chem. 271:30181.

Alternate Names

Cathepsin P, Cathepsin Z, CTSZ

Entrez Gene IDs

1522 (Human); 64138 (Mouse)

Gene Symbol

CTSZ

UniProt

Q9WUU7

Additional Cathepsin X/Z/P Products

Product Documents for Mouse Cathepsin X/Z/P Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Cathepsin X/Z/P Antibody

For research use only

Citations
Reviews

Mouse Cathepsin X/Z/P Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1033

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Cathepsin X/Z/P Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Cathepsin X/Z/P Antibody

Cathepsin X/Z/P in Mouse Splenocytes.