Detects mouse Cathepsin X/Z/P in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Cathepsin X/Z/P is observed and less than 2% cross-reactivity with recombinant mouse (rm) Cathepsin-D, rmCathepsin-H, rmCathepsin-L, rmCathepsin‑C, and rmCathepsin-B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Cathepsin X/Z/P Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References

Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
Nagler and Menard (1998) FEBS Lett. 434:135.
Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
Pungercar, et al. (2000) Pflugers Arch. 439:R119.
Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
Menard, et al. (2001) Biol. Chem. 382:839.
Therrien, et al. (2001) Biochemistry 40:2702.
Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
Guncar, et al. (2000) Structure Fold Des. 8:305.
Lustigman, et al. (1996) J. Biol. Chem. 271:30181.

Alternate Names

Cathepsin P, Cathepsin Z, CTSZ

Entrez Gene IDs

1522 (Human); 64138 (Mouse)

Gene Symbol

CTSZ

UniProt

Q9WUU7

Additional Cathepsin X/Z/P Products

Product Documents for Mouse Cathepsin X/Z/P Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse Cathepsin X/Z/P Biotinylated Antibody

For research use only

Citations
Reviews

Mouse Cathepsin X/Z/P Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1033

Key Product Details

Species Reactivity

Validated:

Cited:

Applications