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Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

R&D Systems, Inc. a Bio-Techne Brand

Substrate for ECE-1, ACE, Cathepsin A, Cathepsin X/Z/P, Neprilysin, and Insulysin
Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate
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ES005
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Key Product Details

Source: N/A
Applications: Binding Activity, Bioactivity

Citations for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate (17)

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Customer Reviews for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate (1)

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  • Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate
    Name: Shrinidh Joshi
    Verified Customer | Posted 08/04/2016
    ACE activity was determined following incubation with intramolecularly quenched synthetic ACE specific substrate Mca-RPGFSAFK (Dnp)-OH (R&D systems). In the case of cell lysates, 10 µg of total protein was assayed for activity in a buffer with the following composition: 50 mM MES (4-morpholineethanesulphonic acid), 300 mM NaCl, 10 µm ZnCl2 and 0.01% Triton X-100, pH 6.5. Reaction was initiated by the addition of 5×10−5 M substrate. Where applicable, recombinant enzymes were used at a concentration of 0.01 µg per reaction. The fluorescence measurements were performed in the black microtiter plates (Costar) in a total volume of 100 µl. The plates were read using a fluorescence plate reader SpectraMax M5 (Molecular Devices) at an excitation wavelength 320 nm and emission wavelength 405 nm Fluorescence resulting from the substrate hydrolysis increased with time, and achieved maximum by one h with recombinant enzyme however with cell/tissue lysates the maximum fluorescence was observed by four hours of incubation. Therefore fluorescence recorded at one and four hours of reaction time was taken for calculation of percent enzyme inhibition, when using recombinant enzymes and cell/tissue lysates, respectively. ACE activity was defined as the ACE inhibitor, captopril-sensitive fluorescence, and were expressed as percent inhibition.
    Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate ES005

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FAQs for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

  • Where does Cathepsin A cleave Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate, Catalog # ES005?

    Although the QC assay conditions provided on our recombinant Cathepsin A datasheets should favor carboxypeptidase activity, it is possible that there is more than one site in the ES005 peptide recognized and cleaved by Cathepsin A.  We have not performed verification experiments to confirm the cleavage site preferred in our reaction conditions.

Product Documents for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

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