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Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

Bio-Techne includes R&D Systems | Catalog # ES005

Substrate for ECE-1, ACE, Cathepsin A, Cathepsin X/Z/P, Neprilysin, and Insulysin
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ES005

Key Product Details

Conjugate

7-Methoxycoumarin

Applications

Binding Activity, Bioactivity

Product Specifications

Source


Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Mca: (7-Methoxycoumarin-4-yl)acetyl, Dnp: 2, 4-Dinitrophenyl.

Purity

>95%, by HPLC.

Predicted Molecular Mass

1388 Da

Activity

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence.

It is an excellent substrate for endothelin-converting enzyme-1 (ECE-1) and neprilysin. The cleavage site by ECE-1 is the peptide bond between Ala and Phe (Johnson, G.D. and Ahn, K., 2000, Anal. Biochem. 286:112). The substrate is derived from bradykinin.

It is also an excellent substrate for ACE and active Cathepsin A and X/Z/P.

Formulation, Preparation and Storage

ES005
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light

Background: Protease Substrates

Additional Protease Substrates Products

Product Documents for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

For research use only

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