Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

Citations are publications that use Bio-Techne products. Selected citations for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate include:

• Species: Human
  Sample Types: Recombinant Proteins
  Applications: Bioassay

  J Mett et al. (2021), Medium-Chain Length Fatty Acids Enhance Abeta Degradation by Affecting Insulin-Degrading Enzyme Cells, 10(11).
  PMID:  34831163
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  Applications: Enzyme Assay

  J Lu et al. (2020), High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity bioRxiv, 0(0).
  PMID:  32637947
• Species: Mouse
  Sample Types: Cell Culture Supernates
  Applications: Bioassay

  M Hohl et al. (2020), Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice J. Biol. Chem., 0(0).
  PMID:  32647007
• Species: Mouse
  Sample Types: Tissue Homogenates
  Applications: Bioassay

  C Dalmasso et al. (2019), Female Mice Exposed to Postnatal Neglect Display Angiotensin II-Dependent Obesity-Induced Hypertension J Am Heart Assoc, 8(23):e012309.
  PMID:  31752639
• Species: Mouse
  Sample Types: Cell Lysates
  Applications: Bioassay

  T Hu et al. (2019), Neuroprotection induced by Navbeta2?knockdown in APP/PS1 transgenic neurons is associated with NEP regulation Mol Med Rep, 20(2):2002-2011.
  PMID:  31257483
• Species: Human
  Sample Types: Cell Lysates
  Applications: Bioassay

  CS Lim et al. (2018), The anti-oxidant xanthorrhizol prevents amyloid-?-induced oxidative modification and inactivation of neprilysin Biosci. Rep., 0(0).
  PMID:  29330223
• Species: Bacteria
  Sample Types: Protein
  

  I Sylte et al. (2018), The selectivity of galardin and an azasugar-based hydroxamate compound for human matrix metalloproteases and bacterial metalloproteases PLoS ONE, 13(8):e0200237.
  PMID:  30075004
• Species: Human
  Sample Types: Recombinant Protein
  Applications: Bioassay

  JE Smith-Carp et al. (2018), Functional requirement for human pitrilysin metallopeptidase 1 arginine 183, mutated in amyloidogenic neuropathy Protein Sci., 0(0).
  PMID:  29383861
• Species: Mouse
  Sample Types: Cell Lysates
  Applications: Activity Assay

  MOW Grimm et al. (2017), Vitamin D and Its Analogues Decrease Amyloid-? (A?) Formation and Increase A?-Degradation Int J Mol Sci, 18(12).
  PMID:  29257109
• Species: Human
  Sample Types: Cell Lysates
  

  Lan X et al. (2011), HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes. J. Immunol., 186(12):6925-32.
  PMID:  21551363
• Species: Human
  Sample Types: Tissue Homogenates
  

  Wang S et al. (2010), Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain. J. Neurochem., 115(1):47-57.
  PMID:  20663017
• Species: Mouse
  Sample Types: Cell Lysates
  

  Wendt W et al. (2009), Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. J. Neurochem., 110(6):1931-41.
  PMID:  19627446
• Species: Human
  Sample Types: Tissue Homogenates
  

  Miners JS et al. (2008), Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid. J. Neurosci. Methods, 167(2):229-36.
  PMID:  17904641
• Species: Rat
  Sample Types: BALF
  

  Wosten-van Asperen RM et al. (2007), ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin. Eur. Respir. J., 31(2):363-71.
  PMID:  17959639
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  Wisner A et al. (2006), Human Opiorphin, a natural antinociceptive modulator of opioid-dependent pathways. Proc. Natl. Acad. Sci. U.S.A., 103(47):17979-84.
  PMID:  17101991
• Species: Human
  Sample Types: Serum
  Applications: Enzyme Assay Substrate

  Liu Z et al. (0), Characterization of insulin degrading enzyme and other amyloid-beta degrading proteases in human serum: a role in Alzheimer's disease? Oncogen29(2):329-40.
  PMID:  22232014
• Species: Human
  Sample Types: Peptide
  Applications: Bioassay

  Lu J et al. (0), High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. J Biol Che295(52):18579-18588.
  PMID:  33122196

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• Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

  Name: Shrinidh Joshi
  Verified Customer | Posted 08/04/2016
  ACE activity was determined following incubation with intramolecularly quenched synthetic ACE specific substrate Mca-RPGFSAFK (Dnp)-OH (R&D systems). In the case of cell lysates, 10 µg of total protein was assayed for activity in a buffer with the following composition: 50 mM MES (4-morpholineethanesulphonic acid), 300 mM NaCl, 10 µm ZnCl2 and 0.01% Triton X-100, pH 6.5. Reaction was initiated by the addition of 5×10−5 M substrate. Where applicable, recombinant enzymes were used at a concentration of 0.01 µg per reaction. The fluorescence measurements were performed in the black microtiter plates (Costar) in a total volume of 100 µl. The plates were read using a fluorescence plate reader SpectraMax M5 (Molecular Devices) at an excitation wavelength 320 nm and emission wavelength 405 nm Fluorescence resulting from the substrate hydrolysis increased with time, and achieved maximum by one h with recombinant enzyme however with cell/tissue lysates the maximum fluorescence was observed by four hours of incubation. Therefore fluorescence recorded at one and four hours of reaction time was taken for calculation of percent enzyme inhibition, when using recombinant enzymes and cell/tissue lysates, respectively. ACE activity was defined as the ACE inhibitor, captopril-sensitive fluorescence, and were expressed as percent inhibition.
  

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