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Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody
Catalog # FAB6311P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 71908
Product Summary for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Glu182
Accession # O14763
Ile56-Glu182
Accession # O14763
Specificity
Detects human TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody
MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B PE-conjugated Monoclonal Antibody (Catalog # FAB6311P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line
Sample: MDA‑MB‑231 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using FAB6311P in the following applications:
Published Applications
Read 27 publications using FAB6311P in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1, TNF R family, transmembrane protein which is a receptor for TRAIL (APO2 Ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Product Documents for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody
Product Specific Notices for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody
For research use only
Citations for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody (27)
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Citations are publications that use Bio-Techne products. Selected citations for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryD Bernareggi et al. (2022-04-07), CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity Nature Communications, 2022-013(1):1899.
PMID: 35393416 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryD Raman et al. (2021-11-20), TRAIL sensitivity of nasopharyngeal cancer cells involves redox dependent upregulation of TMTC2 and its interaction with membrane caspase-3 Redox Biology, 2021-148(0):102193.
PMID: 34839142 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryS Martini et al. (2020-10-02), Selinexor Sensitizes TRAIL-R2-Positive TNBC Cells to the Activity of TRAIL-R2xCD3 Bispecific Antibody Cells, 2020-19(10).
PMID: 33023194 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryJ Dittmann et al. (2019-12-12), Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways Cell Death Differ., 2019-10(0).
PMID: 31831875 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryEB Jo et al. (2019-05-24), Combination therapy with c-met inhibitor and TRAIL enhances apoptosis in dedifferentiated liposarcoma patient-derived cells BMC Cancer, 2019-019(1):496.
PMID: 31126284 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Flow Cytometry,Western BlotS Xu et al. (2018-12-07), miR-494 Sensitizes Gastric Cancer Cells to TRAIL Treatment Through Downregulation of Survivin Cell. Physiol. Biochem., 2018-151(5):2212-2223.
PMID: 30537730 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryB Lian et al. (2018-09-26), miR-128 Targets the SIRT1/ROS/DR5 Pathway to Sensitize Colorectal Cancer to TRAIL-Induced Apoptosis Cell. Physiol. Biochem., 2018-049(6):2151-2162.
PMID: 30257253 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryC You et al. (2018-08-23), Imperatorin Targets MCL-1 to Sensitize CD133+ Lung Cancer Cells to ??-T Cell-Mediated Cytotoxicity Cell. Physiol. Biochem., 2018-049(1):235-244.
PMID: 30138935 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryY Zhao et al. (2017-10-26), Kaempferol Sensitizes Human Ovarian Cancer Cells-OVCAR-3 and SKOV-3 to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis via JNK/ERK-CHOP Pathway and Up-Regulation of Death Receptors 4 and 5 Med. Sci. Monit., 2017-123(0):5096-5105.
PMID: 29070784 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryD Jaworska et al. (2017-07-29), Targeting Apoptotic Activity Against Prostate Cancer Stem Cells Int J Mol Sci, 2017-018(8).
PMID: 28758908 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHD Singh et al. (2017-07-17), TRAIL regulatory receptors constrain human hepatic stellate cell apoptosis Sci Rep, 2017-07(1):5514.
PMID: 28717244 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryWarat M et al. (2015-01-08), The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages. Molecules, 2015-020(1):900-12.
PMID: 25580686 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryWarat M et al. (2014-06-27), Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages. Int J Mol Sci, 2014-015(7):11510-22.
PMID: 24979133 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryCalzolari A et al. (2014-04-16), Salinomycin potentiates the cytotoxic effects of TRAIL on glioblastoma cell lines. PLoS ONE, 2014-09(4):e94438.
PMID: 24740347 -
Species: Rat
Sample Types: Whole Cells
Applications: Flow CytometryTang X et al. (2014-02-01), TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells. Anticancer Res, 2014-034(2):729-34.
PMID: 24511006 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKim et al. (2014-01-16), Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Stem Cells Transl Med, 2014-03(2):172-82.
PMID: 24436439 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryGupta et al. (2013-09-27), Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway. J Biol Chem, 2013-0288(45):32343-56.
PMID: 24078627 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Flow CytometryGojo I et al. (2013-02-12), Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia. Clin Cancer Res, 2013-019(7):1838-51.
PMID: 23403629 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHong S et al. (2012-12-19), Smad7 protein induces interferon regulatory factor 1-dependent transcriptional activation of caspase 8 to restore tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. J Biol Chem, 2012-1288(5):3560-70.
PMID: 23255602 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryPeppa et al. (2012-12-17), Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell-mediated deletion. J Exp Med, 2012-1210(1):99-114.
PMID: 23254287 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKalb ML et al. (2012-01-09), TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity. J. Immunol., 2012-0188(4):1583-91.
PMID: 22231699 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometrySzliszka E et al. (2011-07-06), The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells. Int. J. Oncol., 2011-039(4):771-9.
PMID: 21743964 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKang Z et al. (2011-03-08), Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response. Clin Cancer Res, 2011-017(10):3181-92.
PMID: 21385927 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKim SM et al. (2008-12-01), Gene therapy using TRAIL-secreting human umbilical cord blood-derived mesenchymal stem cells against intracranial glioma. Cancer Res., 2008-168(23):9614-23.
PMID: 19047138 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometrySecchiero P et al. (2008-09-04), Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells. Stem Cells, 2008-026(11):2955-63.
PMID: 18772312 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryWu CH et al. (2008-07-01), TRAIL recombinant adenovirus triggers robust apoptosis in multidrug-resistant HL-60/Vinc cells preferentially through death receptor DR5. Hum. Gene Ther., 2008-019(7):731-43.
PMID: 18476767 -
Species: Human
Sample Types: Serum
Applications: Flow CytometryDunn C et al. (2007-03-12), Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage. J. Exp. Med., 2007-0204(3):667-80.
PMID: 17353365
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Customer Reviews for Human TRAIL R2/TNFRSF10B PE‑conjugated Antibody (1)
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