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Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody
Catalog # FAB6311A | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 71908
Product Summary for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Glu182
Accession # O14763
Ile56-Glu182
Accession # O14763
Specificity
Detects human TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody
Detection of TRAIL R2/TNFRSF10B in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B APC-conjugated Monoclonal Antibody (Catalog # FAB6311A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line
Sample: MDA‑MB‑231 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 3 publications using FAB6311A in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Product Documents for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody
Product Specific Notices for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody
For research use only
Citations for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody (3)
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Citations are publications that use Bio-Techne products. Selected citations for Human TRAIL R2/TNFRSF10B APC‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryU Mert et al. (2019-08-14), TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors Cancers (Basel), 2019-011(8).
PMID: 31416165 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Willms et al. (2019-04-04), Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells PLoS ONE, 2019-014(4):e0214847.
PMID: 30947287 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryMeng X et al. (2014-07-25), Poly(ADP-ribose) polymerase inhibitors sensitize cancer cells to death receptor-mediated apoptosis by enhancing death receptor expression. J Biol Chem, 2014-0289(30):20543-58.
PMID: 24895135
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