Human TRAIL R2/TNFRSF10B Antibody

Name: Human TRAIL R2/TNFRSF10B Antibody
Brand: R&D Systems
SKU: MAB6311

Catalog # MAB6311 | R&D Systems, Inc. a Bio-Techne Brand

Citations (21)

Product Datasheet / COA / SDS

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Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 71908

Product Summary for Human TRAIL R2/TNFRSF10B Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Glu182
Accession # O14763

Specificity

Detects human TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human TRAIL R2/TNFRSF10B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: MDA‑MB‑231 human breast cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 21 publications using MAB6311 in the following applications:

Flow Cytometry (20 Publications)
ICC (1 Publication)
IHC-P (1 Publication)

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

Chaudhary, P.M. et al. (1997) Immunity 7:821.
Walczak, H. et al. (1997) EMBO J. 16:5386.
Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

O14763

Product Documents for Human TRAIL R2/TNFRSF10B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TRAIL R2/TNFRSF10B Antibody

For research use only

Citations for Human TRAIL R2/TNFRSF10B Antibody (21)

Showing 1 - 10 of 21 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human TRAIL R2/TNFRSF10B Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

C Boccellato et al. (2021-06-24), Marizomib sensitizes primary glioma cells to apoptosis induced by a latest-generation TRAIL receptor agonist Cell Death & Disease, 2021-012(7):647.
PMID: 34168123
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

M Bachsais et al. (2020-08-03), CD154 inhibits death of T cells via a Cis interaction with the alpha5beta1 integrin PLoS ONE, 2020-015(8):e0235753.
PMID: 32745080
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

D Stöhr et al. (2020-05-20), Stress-induced TRAILR2 expression overcomes TRAIL resistance in cancer cell spheroids Cell Death Differ., 2020-00(0).
PMID: 32433558
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

SH Lee et al. (2016-10-27), Potential Role of CD133 Expression in the Susceptibility of Human Liver Cancer Stem-Like Cells to TRAIL Oncol. Res., 2016-124(6):495-509.
PMID: 28281970
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Takano S et al. (2014-08-25), Interferon-beta inhibits glioma angiogenesis through downregulation of vascular endothelial growth factor and upregulation of interferon inducible protein 10. Int J Oncol, 2014-045(5):1837-46.
PMID: 25175315
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Neumann S et al. (2014-04-24), Dominant negative effects of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptor 4 on TRAIL receptor 1 signaling by formation of heteromeric complexes. J Biol Chem, 2014-0289(23):16576-87.
PMID: 24764293
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

Tang X et al. (2014-02-01), TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells. Anticancer Res, 2014-034(2):729-34.
PMID: 24511006
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Suzuki-Karasaki M et al. (2013-12-10), Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells. Int J Oncol, 2013-144(2):616-28.
PMID: 24337174
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Stenqvist A et al. (2013-11-01), Exosomes secreted by human placenta carry functional Fas ligand and TRAIL molecules and convey apoptosis in activated immune cells, suggesting exosome-mediated immune privilege of the fetus. J Immunol, 2013-1191(11):5515-23.
PMID: 24184557
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zhou Y et al. (2013-03-11), Casticin potentiates TRAIL-induced apoptosis of gastric cancer cells through endoplasmic reticulum stress. PLoS ONE, 2013-08(3):e58855.
PMID: 23536831
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Neumann S et al. (2012-08-03), The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity. PLoS ONE, 2012-07(8):e42526.
PMID: 22916132
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012-07-06), TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells. Cancer Gene Ther., 2012-019(9):652-8.
PMID: 22767216
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zauli G et al. (2010-02-01), Dexamethasone counteracts the anti-osteoclastic, but not the anti-leukemic, activity of TNF-related apoptosis inducing ligand (TRAIL). J. Cell. Physiol., 2010-0222(2):357-64.
PMID: 19877156
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Stary G et al. (2009-08-18), Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients. Blood, 2009-0114(18):3854-63.
PMID: 19690337
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Seal S et al. (2008-10-05), Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals. Exp. Hematol., 2008-136(12):1660-72.
PMID: 18838202
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Shankar S et al. (2008-08-01), Diallyl trisulfide increases the effectiveness of TRAIL and inhibits prostate cancer growth in an orthotopic model: molecular mechanisms. Mol. Cancer Ther., 2008-07(8):2328-38.
PMID: 18723480
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Barbarotto E et al. (2008-05-15), Differential effects of chemotherapeutic drugs versus the MDM-2 antagonist nutlin-3 on cell cycle progression and induction of apoptosis in SKW6.4 lymphoblastoid B-cells. J. Cell. Biochem., 2008-0104(2):595-605.
PMID: 18092340
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Toscano F et al. (2008-03-17), P53-mediated upregulation of DcR1 impairs oxaliplatin/TRAIL-induced synergistic anti-tumour potential in colon cancer cells. Oncogene, 2008-027(30):4161-71.
PMID: 18345033
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Weinmann L et al. (2008-01-18), A novel p53 rescue compound induces p53-dependent growth arrest and sensitises glioma cells to Apo2L/TRAIL-induced apoptosis. Cell Death Differ., 2008-015(4):718-729.
PMID: 18202704
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Dunn C et al. (2007-03-12), Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage. J. Exp. Med., 2007-0204(3):667-80.
PMID: 17353365
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Singh TR et al. (2005-07-07), HDAC inhibitors enhance the apoptosis-inducing potential of TRAIL in breast carcinoma. Oncogene, 2005-024(29):4609-23.
PMID: 15897906

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