Human TIM-1/KIM-1/HAVCR Antibody

Name: Human TIM-1/KIM-1/HAVCR Antibody
Brand: R&D Systems
SKU: MAB1750
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1750

Citations (17)

(4)

Product Datasheet / COA / SDS

Clone 219211 was used by HLDA to establish CD designation

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR Ser21-Thr288 Accession # AAC39862
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 219211
Specificity:	Detects human TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.

View Full Technical Details for Human TIM-1/KIM-1/HAVCR Antibody

Applications for Human TIM-1/KIM-1/HAVCR Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human TIM-1/KIM-1/HAVCR Antibody

	Western Blot Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of human CD4⁺cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
	Immunohistochemistry TIM‑1/KIM‑1/HAVCR in Human Kidney. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM-1/ KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of
TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

Item	Value
References	Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. Feigelstock, D. et al. (1998) J. Virol. 72:6621. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982. Zhu, C. et al. (2005) Nat. Immunol. 6:1245. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name	T Cell Immunoglobulin Mucin-1
Entrez Gene IDs	26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names	CD365, HAVCR-1, HAVCR1, HAVCRT cell immunoglobin domain and mucin domain protein 1, KIM-1, KIM1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1, TIM-1, TIM-1TIM, TIM1, TIM1TIMD-1, TIMD1T-cell membrane protein 1, hepatitis A virus cellular receptor 1

Product Specific Notices for Human TIM-1/KIM-1/HAVCR Antibody

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

Citations for Human TIM-1/KIM-1/HAVCR Antibody (17)

Showing 1 - 10 of 17 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human TIM-1/KIM-1/HAVCR Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

L Chen et al. (2022), TIM-1 promotes proliferation and metastasis, and inhibits apoptosis, in cervical cancer through the PI3K/AKT/p53 pathway BMC Cancer, 22(1):370.
PMID: 35392845
Species: Human
Sample Types: Whole Cells
Applications: ICC

D Dabbagh et al. (2021), Identification of the SHREK family of proteins as broad-spectrum host antiviral factors bioRxiv : the preprint server for biology, 0(0).
PMID: 33564770
Species: Human
Sample Types: Whole Cells
Applications: Blocking

A Herrador et al. (2019), Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus MBio, 10(2).
PMID: 30914516
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

O Maydan et al. (2018), Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy Physiol Rep, 6(6):e13654.
PMID: 29595914
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Niu et al. (2018), TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection Viruses, 10(11).
PMID: 30441759
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Das et al. (2017), TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions MBio, 8(5).
PMID: 28874468
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

H Fan et al. (2017), Attachment and Post-Attachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission J. Virol., 0(0).
PMID: 28404852
Species: Human
Sample Types: Whole Cells
Applications: ICC

KM Aagaard et al. (2017), Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication Sci Rep, 7(0):41389.
PMID: 28128342
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Patrick Younan et al. (2017), Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm. MBio, 0(0):2150-7511.
PMID: 28951472
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Guangxiang Luo (2016), TIM-1 promotes hepatitis C virus cell attachment and infection J. Virol., 0(0).
PMID: 27807228
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Expression of Tim-1 in primary CNS lymphoma Cancer Med, 5(11):3235-3245.
PMID: 27709813
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Wilkinson R et al. (2014), Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease. PLoS ONE, 9(1):e87345.
PMID: 24475278
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P

Todorovic et al. (2014), Acute pretreatment with chloroquine attenuates renal I/R injury in rats. PLoS ONE, 9(3):e92673.
PMID: 24681567
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kuroda M et al. (2014), A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection. Biochem Biophys Res Commun, 455(3):223-8.
PMID: 25449273
Jemielity et al. (2013), TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine. PLoS Pathog, 9(3):e1003232.
PMID: 23555248
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA

Ivie SE et al. (2011), Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin. PLoS ONE, 6(3):e17787.
PMID: 21412435
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Idali F et al. (2009), Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis. Respir Res, 10(0):42.
PMID: 19480659

There are no citations that match your criteria.

Showing 1 - 10 of 17 citations Showing All

Customer Reviews for Human TIM-1/KIM-1/HAVCR Antibody (4)

4.8 out of 5

4 customer ratings

5 stars

75%

4 stars

25%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 4 of 4 reviews Showing All

Filter By:

Human TIM-1/KIM-1/HAVCR Antibody

Name: Anonymous

Application: WB

Sample Tested: A549 human lung carcinoma cell line,HEK293T human embryonic kidney cell line

Species: Human

Verified Customer | Posted 04/08/2022
Human TIM-1/KIM-1/HAVCR Antibody

Name: Anonymous

Application: IHC

Sample Tested: Kidney tissue

Species: Human

Verified Customer | Posted 11/22/2021
Human TIM-1/KIM-1/HAVCR Antibody

Name: Anonymous

Application: WB

Sample Tested: B cells

Species: Human

Verified Customer | Posted 11/08/2021
Human TIM-1/KIM-1/HAVCR Antibody

Name: Anonymous

Application: IHC

Sample Tested: Kidney tissue

Species: Human

Verified Customer | Posted 08/27/2021

There are no reviews that match your criteria.

Showing 1 - 4 of 4 reviews Showing All

Product Documents for Human TIM-1/KIM-1/HAVCR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human TIM-1/KIM-1/HAVCR Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human TIM-1/KIM-1/HAVCR Antibody

Scientific Data Examples for Human TIM-1/KIM-1/HAVCR Antibody

Western Blot

Flow Cytometry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: TIM-1/KIM-1/HAVCR

Product Specific Notices for Human TIM-1/KIM-1/HAVCR Antibody

Citations for Human TIM-1/KIM-1/HAVCR Antibody (17)

Customer Reviews for Human TIM-1/KIM-1/HAVCR Antibody (4)

Customer Images

Human TIM-1/KIM-1/HAVCR Antibody

Human TIM-1/KIM-1/HAVCR Antibody

Human TIM-1/KIM-1/HAVCR Antibody

Human TIM-1/KIM-1/HAVCR Antibody

Product Documents for Human TIM-1/KIM-1/HAVCR Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected