Human PD-L1/B7-H1 Antibody

Name: Human PD-L1/B7-H1 Antibody
Brand: R&D Systems
SKU: AF156
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF156

Citations (15)

(3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1 Phe19-Thr239 Accession # Q9NZQ7
Applications:	ELISA, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human PD-L1/B7-H1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse PD-L1/B7-H1 is observed and less than 1% cross‑reactivity with recombinant human PD-L2 is observed.

View Full Technical Details for Human PD-L1/B7-H1 Antibody

Applications for Human PD-L1/B7-H1 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	50 µg/mL	See below
ELISA	This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PD-L1/B7-H1 DuoSet ELISA Kit (Catalog # ) for convenient development of a sandwich ELISA or the Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit (Catalog # ) for a complete optimized ELISA.
Immunohistochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human PD-L1/B7-H1 Antibody

	Western Blot Detection of Human PD-L1/B7-H1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry PD-L1/B7-H1 in Human Colon and Colon Cancer Tissue. PD-L1/B7-H1 was detected in immersion fixed paraffin-embedded sections of normal human colon (left panel) and human colon cancer tissue (right panel) using Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	ELISA Human PD-L1/B7-H1 ELISA Standard Curve. Recombinant Human PD-L1/B7-H1 protein was serially diluted 2-fold and captured by Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
	Simple Western Detection of Human PD-L1/B7-H1 by Simple Western^TM. Simple Western lane view shows lysates of HDLM-2 human Hodgkin's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PD-L1/B7-H1 at approximately 72 kDa (as indicated) using 50 µg/mL of Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PD-L1/B7-H1

Human B7 homolog 1 (B7-H1), also called programmed cell death 1 ligand 1 (PDCD1L1) and programmed death ligand 1 (PDL1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 290 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Item	Value
References	Nishimura, H. and T. Honjo (2001) Trends in Immunology 22:265. Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Citations for Human PD-L1/B7-H1 Antibody (15)

Showing 1 - 10 of 15 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human PD-L1/B7-H1 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Z Chu et al. (2022), STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming Nature Communications, 13(1):1859.
PMID: 35388001
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

JJ Park et al. (2021), Checkpoint inhibition through small molecule-induced internalization of programmed death-ligand 1 Nature Communications, 12(1):1222.
PMID: 33619272
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

A Eljaafari et al. (2021), Adipose-Tissue-Derived Mesenchymal Stem Cells Mediate PD-L1 Overexpression in the White Adipose Tissue of Obese Individuals, Resulting in T Cell Dysfunction Cells, 10(10).
PMID: 34685625
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Y Tago et al. (2021), Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferation Scientific Reports, 11(1):2406.
PMID: 33510297
Species: Human
Sample Types: Whole Tissue
Applications: IHC

B Cherif et al. (2021), Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer Scientific Reports, 11(1):7550.
PMID: 33824367
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

R Deng et al. (2020), The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/&beta-catenin signaling pathway Cell. Mol. Immunol., 0(0).
PMID: 32963356
Species: Feline
Sample Types: Whole Cells
Applications: Flow Cytometry

N Taechangam et al. (2019), Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation Stem Cell Res Ther, 10(1):188.
PMID: 31238978
Species: Human
Sample Types: Whole Cells
Applications: Inhibition

LK Thampy et al. (2018), Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration PLoS ONE, 13(6):e0199497.
PMID: 29944697
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

M Idelson et al. (2018), Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Stem Cell Reports, 0(0).
PMID: 30122442
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

S Xue et al. (2017), Blocking the PD-1/PD-L1 pathway in glioma: a potential new treatment strategy J Hematol Oncol, 10(1):81.
PMID: 28388955
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Zhai et al. (2017), Hepatitis C Virus Induces MDSCs-Like Monocytes through TLR2/PI3K/AKT/STAT3 Signaling PLoS ONE, 12(1):e0170516.
PMID: 28114346
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation

(2016), Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression Stem Cells, 0(0).
PMID: 27671847
Species: Human
Sample Types: Serum
Applications: ELISA Development

Melendreras S et al. (2014), Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients. PLoS ONE, 9(12):e113396.
PMID: 25478957
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Jang BC et al. (2007), 12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells. Int. J. Oncol., 31(6):1439-47.
PMID: 17982670
Species: Human
Sample Types: Serum
Applications: ELISA Development

Wan B et al. (2006), Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis. J. Immunol., 177(12):8844-50.
PMID: 17142787

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Customer Reviews for Human PD-L1/B7-H1 Antibody (3)

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Human PD-L1 Antibody

Name: Anonymous

Application: WB

Sample Tested: MDA-MB-231 human breast cancer cell line

Species: Human

Verified Customer | Posted 03/13/2019
Human PD-L1 Antibody

Name: Anonymous

Application: WB

Sample Tested: A549 human lung carcinoma cell line,MDA-MB-231 human breast cancer cell line

Species: Human

Verified Customer | Posted 10/17/2018
Human PD-L1/B7-H1 Antibody

Name: Dong Hu

Application: WB

Sample Tested: MDA-MB-231 human breast cancer cell line

Species: Human

Verified Customer | Posted 05/10/2018

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Human PD-L1/B7-H1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human PD-L1/B7-H1 Antibody

Scientific Data Examples for Human PD-L1/B7-H1 Antibody

Western Blot

Immunohistochemistry

ELISA

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: PD-L1/B7-H1

Citations for Human PD-L1/B7-H1 Antibody (15)

Customer Reviews for Human PD-L1/B7-H1 Antibody (3)

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Human PD-L1 Antibody

Human PD-L1 Antibody

Human PD-L1/B7-H1 Antibody

Product Documents for Human PD-L1/B7-H1 Antibody

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