Human PD-L1/B7-H1 Biotinylated Antibody

Name: Human PD-L1/B7-H1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF156
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF156

Citations (4)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1 Phe19-Thr239 Accession # Q9NZQ7
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human PD-L1/B7-H1 extracellular domain in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) B7RP-1, rhB7‑1, rhB7‑2 is observed.

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Applications for Human PD-L1/B7-H1 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human PD-L1/B7-H1 Fc Chimera (Catalog # 156-B7)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PD-L1/B7-H1

Human B7 homolog 1 (B7-H1), also called programmed cell death 1 ligand 1 (PDCD1L1) and programmed death ligand 1 (PDL1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 290 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Item	Value
References	Nishimura, H. and T. Honjo (2001) Trends in Immunology 22:265. Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Citations for Human PD-L1/B7-H1 Biotinylated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human PD-L1/B7-H1 Biotinylated Antibody include:

Species: Human
Sample Types: Plasma
Applications: ELISA Detection

CP Mao et al. (2021), Protein detection in blood with single-molecule imaging Science Advances, 7(33).
PMID: 34380620
Sample Types: Recombinant Protein
Applications: ELISA Development (Detection)

R Ravi et al. (2018), Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGF? enhance the efficacy of cancer immunotherapy Nat Commun, 9(1):741.
PMID: 29467463
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

J Zhou et al. (2017), Soluble PD-L1 as a biomarker in malignant melanoma and checkpoint blockade Cancer Immunol Res, 0(0).
PMID: 28522460
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry

Xu H et al. (2010), Increased B7-H1 expression on dendritic cells correlates with programmed death 1 expression on T cells in simian immunodeficiency virus-infected macaques and may contribute to T cell dysfunction and disease progression. J. Immunol., 185(12):7340-8.
PMID: 21059890

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Customer Reviews for Human PD-L1/B7-H1 Biotinylated Antibody (1)

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Human PD-L1/B7-H1 Biotinylated Antibody

Name: Leslie Priddy

Application: IHC

Sample Tested: Tumor cell lyastes

Species: Human

Verified Customer | Posted 04/13/2018

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Product Documents for Human PD-L1/B7-H1 Biotinylated Antibody

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Human PD-L1/B7-H1 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human PD-L1/B7-H1 Biotinylated Antibody

Formulation, Preparation and Storage

Reconstitution Calculator

Background: PD-L1/B7-H1

Citations for Human PD-L1/B7-H1 Biotinylated Antibody (4)

Customer Reviews for Human PD-L1/B7-H1 Biotinylated Antibody (1)

Human PD-L1/B7-H1 Biotinylated Antibody

Product Documents for Human PD-L1/B7-H1 Biotinylated Antibody

Product Datasheets

COA

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