Human PD-L1 DuoSet ELISA

Name: Human PD-L1 DuoSet ELISA
Brand: R&D Systems
SKU: DY156
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY156

Citations (8)

(1)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156.25 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human PD-L1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human PD-L1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PD-L1 / B7-H1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human PD-L1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human PD-L1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (R&D Systems®, Catalog # DY005).

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human PD-L1 DuoSet ELISA

	Human PD-L1 / B7-H1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Item	Value
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Citations for Human PD-L1 DuoSet ELISA (8)

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Citations are publications that use Bio-Techne products. Selected citations for Human PD-L1 DuoSet ELISA include:

Species: Human
Sample Types: Plasma

M Roksandic et al. (2022), Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis European review for medical and pharmacological sciences, 26(3):927-934.
PMID: 35179759
Species: Human
Sample Types: Plasma

K Krueger et al. (2022), Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood Biomedicines, 10(10).
PMID: 36289796
Species: Human
Sample Types: Plasma

K Krueger et al. (2022), High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood Biomedicines, 10(10).
PMID: 36289665
Species: Human
Sample Types: Plasma

G Larrinaga et al. (2021), Soluble PD-L1 Is an Independent Prognostic Factor in Clear Cell Renal Cell Carcinoma Cancers, 13(4).
PMID: 33562338
Species: Human
Sample Types: Cell Culture Supernates

C Voisin et al. (2020), Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation? J Clin Med, 9(2).
PMID: 32033151
Species: Human
Sample Types: Serum

S Kruger et al. (2017), Serum levels of soluble programmed death protein 1 (sPD-1) and soluble programmed death ligand 1 (sPD-L1) in advanced pancreatic cancer Oncoimmunology, 6(5):e1310358.
PMID: 28638732
Species: Human
Sample Types: Cell Culture Supernates

(2016), Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression Stem Cells, 0(0).
PMID: 27671847
Species: Human
Sample Types: Plasma

Jovanovic D et al. (0), Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. J Thorac Di10(12):6660-6669.
PMID: 30746211

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Customer Reviews for Human PD-L1 DuoSet ELISA (1)

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Human PD-L1/B7-H1 DuoSet ELISA

Name: Leslie Priddy

Sample Tested: Serum

Verified Customer | Posted 04/04/2018

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Product Documents for Human PD-L1 DuoSet ELISA

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