Human/Mouse/Rat p38 alpha Antibody

Catalog # AF8691 | R&D Systems, Inc. a Bio-Techne Brand
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AF8691
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Summary for Human/Mouse/Rat p38 alpha Antibody

Immunogen

E. coli-derived recombinant human p38 alpha
Met1-Ser360
Accession # Q16539

Specificity

Detects human, mouse and rat p38 alpha. Does not detect recombinant human p38 beta, p38 gamma or p38 delta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat p38 alpha Antibody

Detection of Human, Mouse, and Rat p38a antibody by Western Blot.

Detection of Human, Mouse, and Rat p38 alpha by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38a was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human p38a antibody by Western Blot.

Detection of Human p38 alpha by Western Blot.

Western blot shows recombinant human p38 beta, p38 gamma, p38 delta and Recombinant Human Active p38 alpha (Catalog # 5477-KS) (2 ng/lane). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Orexin B, p38a, & Integrin beta 1 antibody in Mouse Brainstem by Immunohistochemistry (IHC-Fr).

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.

Orexin B, p38a, and Integrin beta1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin beta1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Applications for Human/Mouse/Rat p38 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brainstem

Knockout Validated

p38 alpha is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in p38 alpha knockout HEK293T cell line.

Simple Western

5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using AF8691 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen and protein A Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 alpha

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Alternate Names

MAPK14, SAPK2a

Entrez Gene IDs

1432 (Human); 26416 (Mouse); 81649 (Rat)

Gene Symbol

MAPK14

UniProt

Q16539

Product Documents for Human/Mouse/Rat p38 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p38 alpha Antibody

For research use only

Citations for Human/Mouse/Rat p38 alpha Antibody (6)

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Customer Reviews for Human/Mouse/Rat p38 alpha Antibody (3)

5 out of 5
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  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/17/2022
    Very good detection peaks while using Simple Western, as indicated.
    Human/Mouse/Rat p38 alpha Antibody AF8691
  • Human/Mouse/Rat p38 alpha Antibody
    Name: Haiquan Lu
    Application: Western Blot
    Sample Tested: Human breast cancer cells
    Species: Human
    Verified Customer | Posted 10/24/2018
    Human/Mouse/Rat p38 alpha Antibody AF8691
  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/17/2018
    Human/Mouse/Rat p38 alpha Antibody AF8691

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