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Mouse/Rat Integrin beta 1/CD29 Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # AF2405
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Key Product Details
| Species Reactivity: | Mouse, Rat |
| Immunogen: |
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1 Gln21-Ala738 Accession # P09055 |
| Applications: | CyTOF-ready, Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Polyclonal Goat IgG |
| Specificity: |
Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed. |
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Western BlotDetection of Mouse and Rat Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-3 human prostate cancer cell line, Rat-2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunohistochemistryOrexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
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Simple WesternDetection of Mouse and Rat Integrin beta 1/CD29 by Simple WesternTM. Simple Western lane view shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Mouse/Rat Integrin beta 1/CD29 Antibody (10)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat Integrin beta 1/CD29 Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IFY Xue et al. (2022-01-01), Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth Science Advances, 8(17):eabl8698.
PMID: 35476447 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Heinrich et al. (2021-01-01), Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell Reports, 37(4):109885.
PMID: 34706238 -
Species: Mouse
Sample Types: Testes
Applications: IFA Heinrich et al. (2020-01-01), Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis Cell Rep, 31(2):107513.
PMID: 32294451 -
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow CytometryS Abbasi et al. (2020-01-01), Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 27(3):396-412.e6.
PMID: 32755548 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCUH Langen et al. (2017-01-01), Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 19(3):189-201.
PMID: 28218908 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrWu et al. (2014-01-01), Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice. Gene Ther, 21(9):785-93.
PMID: 24942628 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ImmunoprecipitationConant K et al. (2011-01-01), Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5. J. Neurochem., 118(4):521-32.
PMID: 21166806 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotLiu S et al. (2009-01-01), Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model. Arthritis Rheum., 60(9):2817-21.
PMID: 19714619 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCGendronneau G et al. (2008-01-01), Galectin-7 in the control of epidermal homeostasis after injury. Mol. Biol. Cell, 19(12):5541-9.
PMID: 18829868 -
Species: Rat
Sample Types: Cell Lysates
Applications: ImmunoprecipitationChang Y et al. (2007-01-01), Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis. J. Cell. Sci., 120(0):3053-63.
PMID: 17684062
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