Human Orexin B/Hypocretin‑2 Antibody

Catalog # MAB734 | R&D Systems, Inc. a Bio-Techne Brand
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MAB734
MAB734-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 145202

Product Summary for Human Orexin B/Hypocretin‑2 Antibody

Immunogen

KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide
Accession # O43612

Specificity

Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Orexin B/Hypocretin‑2 Antibody

Orexin B antibody in Mouse Hypothalamus by Immunohistochemistry (IHC-Fr).

Orexin B in Mouse Hypothalamus.

Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Orexin B, p38a, & Integrin beta 1 antibody in Mouse Brainstem by Immunohistochemistry (IHC-Fr).

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.

Orexin B, p38a, and Integrin beta1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human Orexin B/Hypocretin‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brainstem and hypothalamus
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin B/Hypocretin-2

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein (Accession # O43612). Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism.

Alternate Names

Hcrt2, Hypocretin-2, Hypocretin2, PPORX, PPOX

Entrez Gene IDs

3060 (Human)

Gene Symbol

HCRT

UniProt

O43612

Product Documents for Human Orexin B/Hypocretin‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Orexin B/Hypocretin‑2 Antibody

For research use only

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