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Rabbit IgG VisUCyte HRP Polymer Antibody

Bio-Techne includes R&D Systems | Catalog # VC003

Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry

Label

HRP Polymer

Antibody Source

Polyclonal Goat IgG

Product Summary for Rabbit IgG VisUCyte HRP Polymer Antibody

Immunogen

Rabbit IgG

Specificity

Detects heavy ( gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rabbit serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG VisUCyte HRP Polymer Antibody

Detection of SKG in Human Kidney.

Detection of SKG in Human Kidney.

SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog AF3200) at 3 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Applications for Rabbit IgG VisUCyte HRP Polymer Antibody

Application
Recommended Usage

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using VC003 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS with preservative.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG VisUCyte HRP Polymer Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rabbit IgG VisUCyte HRP Polymer Antibody

For research use only

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FAQs for Rabbit IgG VisUCyte HRP Polymer Antibody

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  • Q: What is the recommended procedure for reducing background on tissue sample which appears overstained after using R&D Systems VisUCyte™ HRP polymer?
     

    A: R&D Systems recommend reducing the concentration of the primary antibody, and/or shortening the amount of time the tissue is incubated with our VisUCyte™ HRP polymer reagent.

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