Human SGK1 Antibody

Detection of Human SGK1 by Western Blot

SGK1 inhibits PFV replication. (A to C) HT1080-Control and HT1080 stable expression cells (1 × 105) were infected with PFV (MOI = 0.6). At 48 h postinfection, 600 μL of the supernatants (A) or 1/10 infected HT1080 cell lines (B) were incubated with PFVL cells (1 × 105), the luciferase activity was measured 48 h later, and the rest of the infected cells were lysed for Western blotting (C). (D to F) HT1080-siControl and HT1080-siSGK1 cells (1 × 105) were infected with PFV (MOI = 0.6). At 24 h postinfection, 600 μL of the supernatants (D) or 1/10 infected HT1080 cells (E) were incubated with PFVL cells (1 × 105), and the luciferase activity was measured 48 h later. (F) The rest of the infected cells were lysed for Western blotting. (G to I) PFV (MOI = 0.6) infected Control and sg3 cell lines (1 × 105). At 48 h postinfection, 600 μL of the supernatants (G) or 1/10 infected HT1080 cells (H) were incubated with PFVL cells (1 × 105), and the luciferase activity was measured 48 h later. (I) The remaining infected cells were lysed for Western blotting. Data are expressed as the means ± standard deviations. Data are representative of three independent experiments. One-way ANOVA was used to perform the statistical test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35438526), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human SGK1 by Western Blot

SGK1 inhibits Tas from transactivating PFV LTR and IP promoters. Levels of integrated proviral DNA were measured using semiquantitative PCR (A) and real-time PCR (B). (C) HT1080 cells (1 × 105) were transfected with LTR-Luc (0.05 μg) or IP-Luc (0.025 μg), combined with 3.1-Tas and empty vector or SGK1. At the same time, pCMV-beta -gal (0.05 μg) was transfected to normalize the transfection efficiency. At 48 h posttransfection, luciferase activities were measured and corrected by beta-gal catalytic activities. The remaining cell lysates were used for Western blotting. (D) HT1080-siControl and HT1080-siSGK1 cells (1 × 105) were transfected with LTR-Luc (0.05 μg) or IP-Luc (0.025 μg) and 3.1-Tas. At the same time, pCMV-beta -gal (0.05 μg) was transfected to normalize transfection efficiency. At 48 h posttransfection, luciferase activities were measured and corrected by beta-gal catalytic activities. The remaining cell lysates were used for Western blotting. Data are expressed as the means ± standard deviations. Data are representative of three independent experiments. One-way ANOVA was used to perform the statistical test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; and ns for P > 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35438526), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human SGK1 by Western Blot

SGK1 inhibits the transactivation function of Tas in a kinase-independent manner. (A to C) HEK293T cells (2 × 105) were co-transfected with pcPFV (0.5 μg) and empty vector, S422D, SGK1, or K127N (0.5 μg). At 48 h posttransfection, 600 μL of the supernatants (A) or 1/20 transfected cells (B) were incubated with PFVL cells (1 × 105), the luciferase activity was measured 48 h later. (C) The rest of transfected cells were lysed for Western blotting. (D, E) HEK293T cells (2 × 105) were transfected with LTR-Luc (0.025 μg) (D) or IP-Luc (0.01 μg) (E), combined with 3.1-Tas (0.1 μg) and empty vector or SGK1, SGK1-S422D, and SGK1-K127N (0.3 μg). At the same time, pCMV-beta -gal (0.025 μg) was transfected to normalize the transfection efficiency. At 48 h posttransfection, luciferase activities were measured and corrected by beta-gal catalytic activities. Remaining cell lysates were used for Western blotting. Data are expressed as the means ± standard deviations. Data are representative of three independent experiments. One-way ANOVA was used to perform the statistical test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns for P > 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35438526), licensed under a CC-BY license. Not internally tested by R&D Systems.