Human SGK1 Alexa Fluor® 750-conjugated Antibody

Name: Human SGK1 Alexa Fluor® 750-conjugated Antibody
Brand: R&D Systems
SKU: AF3200S

R&D Systems, part of Bio-Techne | Catalog # AF3200S

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human SGK1 Met1-Leu431 Accession # O00141
Applications:	Immunohistochemistry, Western Blot
Label:	Alexa Fluor 750 (Excitation=749 nm, Emission=775 nm)
Specificity:	Detects human SGK1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SGK2 and rhSGK3 is observed.

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Technical Data
Citations / Reviews

Applications for Human SGK1 Alexa Fluor® 750-conjugated Antibody

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: SGK1

Serum- and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases. In addition to serum and glucocorticoids, insulin, IGF-I, osmotic shock, and mineralocorticoids have been demonstrated to activate SGK1. Expressed at low levels, SGK1 appears to function as a regulator of epithelial ion transport. Sustained high levels of SGK1 activation have been implicated in hypertension and diabetic nephropathy.

Item	Value
Long Name	Serum/Glucocorticoid Regulated Kinase 1
Entrez Gene IDs	6446 (Human); 20393 (Mouse); 29517 (Rat)
Alternate Names	EC 2.7.11, EC 2.7.11.1, SGK, SGK1, Serum/glucocorticoid-regulated kinase 1, serine/threonine protein kinase SGK, serine/threonine-protein kinase Sgk1, serum/glucocorticoid regulated kinase, serum/glucocorticoid regulated kinase 1

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