Human IL-27 Antibody

Name: Human IL-27 Antibody
Brand: R&D Systems
SKU: AF2526
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2526

Citations (11)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑27 (R&D Systems, Catalog # 2526-IL) Arg21-Lys229 (IL-27 EBI-3 subunit), Phe29-Pro243 (IL-27 p28 subunit) Accession # Q14213 (EBI-3 subunit), AAM34498 (p28 subunit)
Applications:	Block/Neutralize, ELISA Capture, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL-27 in ELISAs and Western blots.

View Full Technical Details for Human IL-27 Antibody

Applications for Human IL-27 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below

Human IL-27 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Human IL‑27 Biotinylated Antibody (Catalog # BAF2526)

Standard: Recombinant Human IL-27 Protein (Catalog # 2526-IL)

Item	Value
Neutralization	Measured by its ability to neutralize IL‑27 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Bender, H. et al. (2009) Hepatology 50:585. The Neutralization Dose (ND₅₀) is typically 0.1‑0.8 µg/mL in the presence of 25 ng/mL Recombinant Human IL‑27.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-27 Antibody

	Western Blot Detection of Human IL‑27 by Western Blot. Western blot shows lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Neutralization IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IL‑27 Antibody. Recombinant Human IL-27 (Catalog # 2526-IL) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-27 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526). The ND₅₀ is typically 0.1-0.8 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), also known as IL27B, a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, also known as IL27A, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti‑inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL‑12 receptor on naïve CD4⁺ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

Item	Value
References	Boulay, J-L. et al. (2003) Immunity 19:159. Trinchieri, G. et al. (2003) Immunity 19:641. Murakami, M. et al. (2004) Growth Factors 22:75. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. Devergne, O. et al. (1996) J. Virology 70:1143. Pflanz, S. et al. (2002) Immunity 16:779. Villarino, A.V. et al. (2004) J. Immunol. 173:715. Pflanz, S. et al. (2004) J Immunol 172:2225. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.
Long Name	Interleukin 27
Entrez Gene IDs	246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)
Alternate Names	IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27p28, IL30, MGC71873, interleukin 27, interleukin 30, interleukin-27 subunit alpha, p28IL-27A

Citations for Human IL-27 Antibody (11)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-27 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

XY Zhang et al. (2022), IL-27 promotes decidualization via the STAT3-ESR/PGR regulatory axis Journal of reproductive immunology, 151(0):103623.
PMID: 35430461
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

I Gregersen et al. (2017), Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation PLoS ONE, 12(11):e0188387.
PMID: 29176764
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

A Garg et al. (2017), Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4 Sci Rep, 7(0):44485.
PMID: 28338007
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)

N Gajanayaka et al. (2017), HIV and HIV-Tat inhibit LPS-induced IL-27 production in human macrophages by distinct intracellular signaling pathways J. Leukoc. Biol., 0(0).
PMID: 28698313
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

JK Sprokholt et al. (2017), RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production PLoS Pathog., 13(11):e1006738.
PMID: 29186193
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

G Tan et al. (2017), Type I IFN augments IL-27-dependent TRIM25 expression to inhibit HBV replication Cell. Mol. Immunol, 0(0).
PMID: 28194021
Species: Human
Sample Types: Whole Cells
Applications: IHC

Xie M et al. (2015), IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signaling. J Allergy Clin Immunol, 135(2):386-94.
PMID: 25312760
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Fitzgerald D et al. (2013), Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis. J Immunol, 190(7):3225-34.
PMID: 23455508
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Ramgolam VS et al. (2011), B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis. J. Immunol., 186(0):4518.
PMID: 21368231
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Kalliolias GD et al. (2010), Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27. J. Immunol., 185(11):7047-56.
PMID: 20971923
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Gringhuis S et al. (0), Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production. Nat Commu5(0):5074.
PMID: 25278262

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Human IL-27 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 11/27/2017

AF2526 was used as the solid phase antibody, BAF2526 was used as the detection antibody. 2526-IL was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.

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Human IL-27 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human IL-27 Antibody

Human IL-27 Sandwich Immunoassay

Scientific Data Examples for Human IL-27 Antibody

Western Blot

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-27

Citations for Human IL-27 Antibody (11)

Customer Reviews for Human IL-27 Antibody (1)

Customer Images

Human IL-27 Antibody

Product Documents for Human IL-27 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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