Human IL-27 Biotinylated Antibody

Name: Human IL-27 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2526
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF2526

Citations (3)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑27 (R&D Systems, Catalog # 2526-IL) Arg21-Lys229 (IL-27 EBI-3 subunit), Phe29-Pro243 (IL-27 p28 subunit) Accession # Q14213 (EBI-3 subunit), AAM34498 (p28 subunit)
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL-27 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse IL‑27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed.

View Full Technical Details for Human IL-27 Biotinylated Antibody

Applications for Human IL-27 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below

Human IL-27 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Standard: Recombinant Human IL-27 Protein (Catalog # 2526-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-27 Biotinylated Antibody

	Western Blot Detection of Human IL‑27 by Western Blot. Western blot shows lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-27 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2526) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for the IL-27 EBI3/IL-27B subunit at approximately 32 kDa and IL-27p28/IL27-A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), also known as IL27B, a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, also known as IL27A, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti‑inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL‑12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

Item	Value
References	Boulay, J-L. et al. (2003) Immunity 19:159. Trinchieri, G. et al. (2003) Immunity 19:641. Murakami, M. et al. (2004) Growth Factors 22:75. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. Devergne, O. et al. (1996) J. Virology 70:1143. Pflanz, S. et al. (2002) Immunity 16:779. Villarino, A.V. et al. (2004) J. Immunol. 173:715. Pflanz, S. et al. (2004) J Immunol 172:2225. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.
Long Name	Interleukin 27
Entrez Gene IDs	246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)
Alternate Names	IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27p28, IL30, MGC71873, interleukin 27, interleukin 30, interleukin-27 subunit alpha, p28IL-27A

Citations for Human IL-27 Biotinylated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-27 Biotinylated Antibody include:

Species: Human
Sample Types: Protein
Applications: ELISA Capture

MC Cavé et al. (2020), Glycosaminoglycans bind human IL-27 and regulate its activity Eur. J. Immunol., 0(0).
PMID: 32483835
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection)

Dietrich C et al. (2014), A soluble form of IL-27Ralpha is a natural IL-27 antagonist. J Immunol, 192(11):5382-9.
PMID: 24771852
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Kalliolias GD et al. (2010), Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27. J. Immunol., 185(11):7047-56.
PMID: 20971923

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Human IL-27 Biotinylated Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 11/27/2017

AF2526 was used as the solid phase antibody, BAF2526 was used as the detection antibody. 2526-IL was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.

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Product Documents for Human IL-27 Biotinylated Antibody

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Human IL-27 Biotinylated Antibody

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Applications for Human IL-27 Biotinylated Antibody

Human IL-27 Sandwich Immunoassay

Scientific Data Examples for Human IL-27 Biotinylated Antibody

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-27

Citations for Human IL-27 Biotinylated Antibody (3)

Customer Reviews for Human IL-27 Biotinylated Antibody (1)

Customer Images

Human IL-27 Biotinylated Antibody

Product Documents for Human IL-27 Biotinylated Antibody

Product Datasheets

COA

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