Human GATA‑3 Antibody
Catalog # MAB26052 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human GATA‑3 Antibody
Immunogen
Pro135-Ser258
Accession # P23771
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human GATA‑3 Antibody
Detection of Human GATA‑3 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26052) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Detection of GATA‑3 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th2 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and either (A) Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26052) or (B) Mouse IgG2BFlow Cytometry Isotype Control (MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.Detection of GATA‑3 in MCF-7 cells by Flow Cytometry.
MCF-7 cells were stained with Mouse Anti-Human GATA‑3 Monoclonal Antibody (Catalog # MAB26052, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FC012 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.Applications for Human GATA‑3 Antibody
Intracellular Staining by Flow Cytometry
Sample: Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th2 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012) and MCF‑7 human breast cancer cell line.
Western Blot
Sample: MCF‑7 human breast cancer cell line
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-3
Product Documents for Human GATA‑3 Antibody
Product Specific Notices for Human GATA‑3 Antibody
For research use only
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.