Mouse IgG2B Isotype Control Best Seller

Detection of Mouse IgG Control by Flow Cytometry

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB89001, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).

Detection of IgG2B by Flow Cytometry

Prolonged stimulation through activating receptors induces NK cell exhaustion.(A) Schematic representing the in vitro model of exhaustion. Agonists of NKp46 (anti-NKp46) and NKG2D (MICA and MICB) were adsorbed onto tissue culture plates and used to stimulate NK cells for 7 days. Plate-bound isotype IgG served as a control. Both groups received 1 ng/mL IL-15. (B) NK cells harvested from isotype-coated and exhaustion plates (day 7) were incubated with K-562 targets for 4 hours (E/T: 2:1). Cytokine production (IFN-gamma and TNF-alpha) and degranulation (CD107a) were measured via flow cytometry. Isotype NK cells (top row) in blue, exhausted NK cells (bottom row) in red. E/T, effector/target. (C–E) Quantification of cytokine production and degranulation as percentage of parent population (live, CD3–CD56+ cells) and mean fluorescence intensity (MFI) (n = 4). Paired t tests were used for comparisons. **P < 0.01; ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/150079), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IgG2B by Flow Cytometry

Prolonged stimulation through activating receptors results in functional and proliferative defects in vivo.(A) Schematic representing experimental design: sublethally irradiated NSG mice were injected (i.v.) with HL-60-Luc2 cells and injected (i.v.) with NK cells 3 days later. NK cells had been incubated for 7 days on plates with either isotype IgG (control) or anti-NKp46 and MICA/B as previously described. (B) Fourteen days after NK cell injection, blood was drawn, and NK cells were counted via flow cytometry. NK cells were human CD45+CD56+CD3–. One-way ANOVA was used for comparisons (n = 8). *P ≤ 0.05. (C) HL-60 tumor burden was tracked via bioluminescent imaging (BLI), with day 21 data pictured. One-way ANOVA was used for comparisons (n = 8). ***P < 0.001. (D and E) Fourteen days after NK cell injection, NK cells were restimulated with K-562 leukemia cells for 4 hours as previously described. Ki67, IFN-gamma, TNF-alpha, and CD107a expression was assessed via flow cytometry (D). Paired t tests were used for comparisons (n = 8). *P ≤ 0.05. Representative data depict Ki67 and IFN-gamma expression following K-562 restimulation (E). Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/150079), licensed under a CC-BY license. Not internally tested by R&D Systems.