Key Product Details
Accession # P23771
Scientific Data Images
Detection of GATA‑3 in Human PBMCs by Flow Cytometry.Th2-stimulated human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N) and either (A) Mouse Anti-Human GATA-3 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC63301T) or (B) Mouse IgG2BAlexa Fluor 594 Isotype Control (Catalog # IC0041T). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of GATA‑3 in MCF-7 cell line by Flow Cytometry.MCF-7 human breast carcinoma cell line was stained with Mouse Anti-Human GATA-3 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC63301T, filled histogram) or Mouse IgG2BAlexa Fluor 594 Isotype Control (IC0041T, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.
Intracellular Staining by Flow Cytometry
Sample: Th2-stimulated human peripheral blood mononuclear cells (PBMCs) and MCF-7 human breast carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only