Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Name: Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody
Brand: R&D Systems
SKU: BAM365
Price: 512 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAM365

Citations (5)

Product Datasheet / COA / SDS

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Key Product Details

Species Reactivity:	Human
Immunogen:	NS0-derived and E.coli-derived Recombinant Human Fractalkine Gln25-Arg339 Accession # P78423
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Monoclonal Mouse IgG₁ Clone # 51637
Specificity:	Detects human CX3CL1 in ELISAs and Western blots. In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.

View Full Technical Details for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Applications for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)

Human CX3CL1/Fractalkine Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)

Standard: Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # 365-FR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Item	Value
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

A Babendreye et al. (2017), Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion Mediators Inflamm., 2017(0):1515389.
PMID: 28522896
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection)

N Quillinan et al. (2017), Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Arthritis Res. Ther, 19(1):45.
PMID: 28270187
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Popovic M et al. (2008), Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. J. Leukoc. Biol., 84(1):215-23.
PMID: 18436581
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Hundhausen C et al. (2007), Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J. Immunol., 178(12):8064-72.
PMID: 17548644
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Lavergne E et al. (2003), Fractalkine mediates natural killer-dependent antitumor responses in vivo. Cancer Res., 63(21):7468-74.
PMID: 14612547

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Showing 1 - 5 of 5 citations Showing All

Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Human CX3CL1/Fractalkine Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (5)

Product Documents for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

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