Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

Name: Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody
Brand: R&D Systems
SKU: IC365P
Price: 392 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC365P

Citations (6)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain Gln25-Arg339 (Ser199Asn) (predicted) Accession # P78423
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 51637
Specificity:	Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.

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Applications for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

	Intracellular Staining by Flow Cytometry Detection of CX3CL1/Fractalkine in NS0 Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry. NS0 cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A) and either (A) Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Monoclonal Antibody (Catalog # IC365P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

Item	Value
References	Pan, Y. et al. (1997) Nature 387:611. Bazan, J.F. et al. (1997) Nature 385:640. Mackay, C.R. (1997) Current Biology 7:R384.
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Shin M et al. (2015), DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine. J Immunol, 195(6):2861-9.
PMID: 26276874
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kassianos A et al. (2015), Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells. Kidney Int, 87(6):1153-63.
PMID: 25587706
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Truman LA et al. (2008), CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood, 112(13):5026-36.
PMID: 18799722
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hermand P et al. (2008), Functional adhesiveness of the CX3CL1 chemokine requires its aggregation. Role of the transmembrane domain. J. Biol. Chem., 283(44):30225-34.
PMID: 18725411
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hundhausen C et al. (2007), Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J. Immunol., 178(12):8064-72.
PMID: 17548644
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lavergne E et al. (2003), Fractalkine mediates natural killer-dependent antitumor responses in vivo. Cancer Res., 63(21):7468-74.
PMID: 14612547

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Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

Scientific Data Examples for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody (6)

Product Documents for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody

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