Human CD40/TNFRSF5 Antibody

Name: Human CD40/TNFRSF5 Antibody
Brand: R&D Systems
SKU: MAB6321
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB6321

Citations (6)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5 Glu21-Arg193 Accession # P25942
Applications:	CyTOF-ready, Flow Cytometry, Functional Assay, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 82111
Specificity:	Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse CD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, or rhTNF RII.

View Full Technical Details for Human CD40/TNFRSF5 Antibody

Applications for Human CD40/TNFRSF5 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Human whole blood CD19⁺ B cells
Agonist Activity	Measured in a cell proliferation assay using B cell enriched human peripheral blood lymphocytes in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED₅₀ for this effect is typically 0.035-0.175 μg/mL.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD40/TNFRSF5 Antibody

	Immunocytochemistry CD40/TNFRSF5 in Human PBMCs. CD40/TNFRSF5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD40/TNFRSF5 Monoclonal Antibody (Catalog # MAB6321) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Agonist Activity Human CD40/TNFRSF5 Antibody Stimulates Cell Proliferation in Human B Cells. Mouse Anti-Human CD40/TNFRSF5 Monoclonal Antibody (Catalog # MAB6321) stimulates human B cell proliferation in the presence of Recombinant Human IL-4 (Catalog # 204-IL) in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED₅₀ for this effect is typically 0.035-0.175 µg/mL

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD40/TNFRSF5

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature hCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 62 aa cytoplasmic domain (1). Human and mouse CD40 share 62% aa identity. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by CD40 binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the
CD40‑CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).

Item	Value
References	Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569. Armitage, R.J. et al. (1993) J. Immunol. 150:3671. Callard, R.E. et al. (1993) Immunol. Today 14:559. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487. Pullen, S.S. et al. (1999) Biochemistry 38:10168. Faris, M. et al. (1994) J. Exp. Med. 179:1923. Hanissian, S.H. and R.S Geha (1997) Immunity 6:379.
Entrez Gene IDs	958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)
Alternate Names	B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, TNFRSF5, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), nerve growth factor receptor-related B-lymphocyte activation molecule, p50, tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

Citations for Human CD40/TNFRSF5 Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD40/TNFRSF5 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Stimulation

I Harder et al. (2022), Dysregulated PI3K Signaling in B Cells of CVID Patients Cells, 11(3).
PMID: 35159274
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

G Brummer et al. (2019), CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments Oncogene, 0(0).
PMID: 31827233
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Heine G et al. (2014), Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts. Eur J Immunol, 44(6):1615-21.
PMID: 24643722
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Maddur M et al. (2014), Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand. Nat Commun, 5(0):4092.
PMID: 24910129
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Loebel M et al. (2014), Deficient EBV-specific B- and T-cell response in patients with chronic fatigue syndrome. PLoS ONE, 9(1):e85387.
PMID: 24454857
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Divoux A et al. (2010), Fibrosis in human adipose tissue: composition, distribution, and link with lipid metabolism and fat mass loss. Diabetes, 59(11):2817-25.
PMID: 20713683

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Human CD40/TNFRSF5 Antibody

Name: Anonymous

Application: WB

Sample Tested: Cancer cell lysates,Min6 mouse insulinoma whole cell lysate

Species: Human

Verified Customer | Posted 06/02/2019

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Human CD40/TNFRSF5 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CD40/TNFRSF5 Antibody

Scientific Data Examples for Human CD40/TNFRSF5 Antibody

Immunocytochemistry

Agonist Activity

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD40/TNFRSF5

Citations for Human CD40/TNFRSF5 Antibody (6)

Customer Reviews for Human CD40/TNFRSF5 Antibody (1)

Customer Images

Human CD40/TNFRSF5 Antibody

Product Documents for Human CD40/TNFRSF5 Antibody

Product Datasheets

COA

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