Human CD40/TNFRSF5 Antibody

Name: Human CD40/TNFRSF5 Antibody
Brand: R&D Systems
SKU: MAB6322

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB6322

Citations (8)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5 Glu21-Arg193 Accession # P25942
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 82102
Specificity:	Detects human CD40 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, or rhTNF RII is observed.

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Applications for Human CD40/TNFRSF5 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below

Neutralization

Measured by its ability to neutralize CD40 Ligand/TNFSF5-induced proliferation in human B cell enriched peripheral blood mononuclear cells (PBMC). At 5 µg/mL, this anti-hCD40 antibody will neutralize approximately 80% of 10 µg/mL Recombinant Human CD40 Ligand/TNFSF5 -induced proliferation of human B-cell enriched PBMCs.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD40/TNFRSF5 Antibody

	Neutralization Cell Proliferation Induced by CD40 Ligand/TNFSF5 and Neutralization by Human CD40/TNFRSF5 Antibody. In the presence of Recombinant Human IL-4 (Catalog # 204-IL), Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) stimulates proliferation in human B cell enriched peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CD40 Ligand/TNFSF5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Human CD40/TNFRSF5 Monoclonal Antibody (Catalog # MAB6322). At 5 µg/mL, this anti-hCD40 antibody will neutralize approximately 80% of Recombinant Human CD40 Ligand/TNFSF5 -induced proliferation in the presence of Recombinant Human IL-4 (20 ng/mL).
	Western Blot Detection of CD40/TNFRSF5 Mouse by Western Blot. Western blot shows lysates of human spleen tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD40/TNFRSF5 Monoclonal Antibody (Catalog # MAB6322) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD40/TNFRSF5 at approximately 40 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD40/TNFRSF5

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. Mature human CD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 62 aa cytoplasmic domain (1). Human and mouse CD40 share 62% aa identity. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by CD40 binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6-8).

Item	Value
References	Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569. Armitage, R.J. et al. (1993) J. Immunol. 150:3671. Callard, R.E. et al. (1993) Immunol. Today 14:559. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487. Pullen, S.S. et al. (1999) Biochemistry 38:10168. Faris, M. et al. (1994) J. Exp. Med. 179:1923. Hanissian, S.H. and R.S Geha (1997) Immunity 6:379.
Entrez Gene IDs	958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)
Alternate Names	B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, TNFRSF5, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), nerve growth factor receptor-related B-lymphocyte activation molecule, p50, tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

Citations for Human CD40/TNFRSF5 Antibody (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD40/TNFRSF5 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Shankar et al. (2022), Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 13(1):3121.
PMID: 35660734
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

FH Al-Awadhi et al. (2018), Structural Diversity and Anticancer Activity of Marine-Derived Elastase Inhibitors: Key Features and Mechanisms Mediating the Antimetastatic Effects in Invasive Breast Cancer Chembiochem, 0(0).
PMID: 29405541
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

C Sun et al. (2017), Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis Cell. Physiol. Biochem., 41(6):2319-2332.
PMID: 28463834
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

E Martin-Gay et al. (2017), Circulating CXCR5(+)CXCR3(+)PD-1(lo) Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth JCI Insight, 2(2):e89574.
PMID: 28138558
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Dietrich C et al. (2014), A soluble form of IL-27Ralpha is a natural IL-27 antagonist. J Immunol, 192(11):5382-9.
PMID: 24771852
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2012), ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 119(26):6268-77.
PMID: 22577174
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

van der Vliet HJ et al. (2008), Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells. J. Immunol., 180(11):7287-93.
PMID: 18490728
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Ettinger R et al. (2007), IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J. Immunol., 178(5):2872-82.
PMID: 17312131

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Showing 1 - 8 of 8 citations Showing All

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Human CD40/TNFRSF5 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human CD40/TNFRSF5 Antibody

Scientific Data Examples for Human CD40/TNFRSF5 Antibody

Neutralization

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD40/TNFRSF5

Citations for Human CD40/TNFRSF5 Antibody (8)

Product Documents for Human CD40/TNFRSF5 Antibody

Product Datasheets

COA

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