Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Name: Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein
Brand: R&D Systems
SKU: 6245-CL
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6245-CL

Citations (6)

(1)

Product Datasheet / COA / SDS

A New rh CD40 Ligand is Available! ~1000 fold better activity; HEK293 expressed; HA tag!

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Key Product Details

Source:	E. coli
Accession #:	P29965
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Product Specifications for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Source	E. coli-derived human CD40 Ligand/TNFSF5 protein Glu108-Leu261, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	16.9 kDa (monomer)
SDS-PAGE	18 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED₅₀ for this effect is 1.00‑3.00 μg/mL in the presence of 20 ng/mL of recombinant human IL‑4.

Scientific Data Examples for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

	Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein Bioactivity Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) (Catalog # 6245-CL) stimulates cell proliferation using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The ED₅₀ for this effect is 1.00-3.00 µg/mL in the presence of 20 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL).
	Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein SDS-PAGE 1 μg/lane of Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

6245-CL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCL, NaCl and EDTA with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.

6245-CL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCL, NaCl and EDTA.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD40 Ligand/TNFSF5

Human CD40 LigandCD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily (1-3). Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region (4, 5). The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms (6-9). The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable (8). CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells (10). The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells (11). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (12, 13). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (14, 15). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (16, 17).

Item	Value
References	Zhang, G. (2004) Curr. Opin. Struct. Biol. 14:154. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Quezada, S.A. et al. (2004) Annu. Rev. Immunol. 22:307. Graf, D. et al. (1992) Eur. J. Immunol. 22:3191. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313. Khandekar, S.S. et al. (2001) Protein Expr. Purif. 23:301. Pietravalle, F. et al. (1996) J. Biol. Chem. 271:5965. Garber, E. et al. (1999) J. Biol. Chem. 274:33545. Vakkalanka, R.K. et al. (1999) Arthritis Rheum. 42:871. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330. Eissner, G. et al. (2004) Cytokine Growth Factor. Rev. 15:353. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115. Elgueta, R. et al. (2009) Immunol. Rev. 229:152. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373. Chougnet, C. (2003) J. Leukoc. Biol. 74:702. Pamukcu, B. et al. (2011) Ann. Med. 43:331. Hassan, G.S. et al. (2012) Immunobiology 217:521.
Entrez Gene IDs	959 (Human); 21947 (Mouse); 84349 (Rat)
Alternate Names	CD154, CD154 antigen, CD40 Ligand, CD40 antigen ligand, CD40-L, CD40L, CD40LG, CD40LIGM, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), Tumor necrosis factor ligand superfamily member 5, gp39, hCD40L, tumor necrosis factor (ligand) superfamily member 5

Citations for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Stimulation

JL Lee et al. (2022), B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction Aging Cell, 0(0):e13692.
PMID: 35980826
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

N Eiza et al. (2022), Truncated-semaphorin3A is a potential regulatory molecule to restore immune homeostasis in immune-mediated diseases Frontiers in Pharmacology, 13(0):1085892.
PMID: 36703747
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Kim et al. (2021), Regulation of B cell functions by S-nitrosoglutathione in the EAE model Redox Biology, 45(0):102053.
PMID: 34175668
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Artyomov M et al. (2015), Modular expression analysis reveals functional conservation between human Langerhans cells and mouse cross-priming dendritic cells. J Exp Med, 212(5):743-57.
PMID: 25918340
Species: Human
Sample Types: Whole Blood
Applications: Bioassay

Singh et al. (2014), Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation. J Immunol, 192(10):4674-84.
PMID: 24729609
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Corvaisier M et al. (2012), IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 10(9):e1001395.
PMID: 23055831

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Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 10/20/2017

Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Scientific Data Examples for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Formulation, Preparation and Storage

Carrier Free

6245-CL

Carrier

6245-CL/CF

Carrier Free

Reconstitution Calculator

Background: CD40 Ligand/TNFSF5

Citations for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein (6)

Customer Reviews for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein (1)

Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Product Documents for Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

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