Thiazovivin

Product Description

Thiazovivin is a selective, cell-permeable Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor (IC₅₀ = 0.5 μM). Thiazovivin enhances the efficiency of fibroblast reprogramming to generate induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. No. 1614) and PD 0325901 (Cat. No. 4192). Thiazovivin improves the survival of human embryonic stem cells (hESCs) upon trypsinization and increases cell adhesion through the regulation of E-cadherin and significantly improves direct reprogramming efficiency. In combination with Purmorphamine (Cat. No. 4551) and A 83-01 (Cat. No. 2939), Thiazovivin can be used to directly reprogram mouse embryonic fibroblasts into chemically induced neural stem cells.

Solubility

Preparing Stock Solutions for Thiazovivin

The following data is based on the product molecular weight 311.36

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.21 mL	16.06 mL	32.12 mL
5 mM	0.64 mL	3.21 mL	6.42 mL
10 mM	0.32 mL	1.61 mL	3.21 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Xu Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc.Natl.Acad.Sci. USA 2010 PMID: 20406903
• Zheng A combination of small molecules directly reprograms mouse fibroblasts into neural stem cells. Biochem.Biophys.Res.Commun. 2016 PMID: 27207831
• Lin A chemical platform for improved induction of human iPSCs. Nat.Methods. 2009 PMID: 19838168