Endothelin 1 (human, porcine)

Name: Endothelin 1 (human, porcine)
Brand: Tocris Bioscience
SKU: 1160

Tocris Bioscience, part of Bio-Techne | Catalog # 1160

Citations (37)

COA / SDS

Potent vasoconstrictor peptide

100% Guarantee

Product Description
Scientific Data
Product Specifications
Solubility
Calculators
Background References
Product Documents
Specific Notices

Key Product Details

Description

Potent vasoconstrictor peptide

Alternative Names

ET-1

View all Non-selective Endothelin Receptor Agonists »

Product Description

Endothelin 1 (human, porcine) is a potent endogenous peptide agonist at ET_A and ET_B receptors. Produces strong vasoconstriction and pressor responses. Competitive binding assay shows that Endothelin 1 reversibly binds to receptors (IC₅₀ values are 0.12 nM and 0.15 nM at ET_B and ET_A receptors respectively). Potency is dependent on blood vessel type (EC₅₀ ranges from 2.72 nM - 17.4 nM). Activates PLC (EC₅₀ = 0.3 nM), increases production of PGE₂ (EC₅₀ = 12 nM) and induces intracellular calcium release in human ciliary muscle cells. Also upregulates leptin mRNA expression and secretion in adipocytes (EC₅₀ = 8.8 nM).

Scientific Data Images for Endothelin 1 (human, porcine)

Pluripotent stem cell-derived cardiomyocytes secrete Pro-BNP in response to small molecule-induction of cardiac hypertrophy

Cardiomyocytes were treated with the vasoconstrictor, ET-1 (Endothelin-1; Catalog # 1160), or one of two L-type calcium channel inhibitors, Doxorubicin (Catalog # 2252) or Verapamil (Catalog # 0654). Levels of the cardiac hypertrophy marker, Pro-Brain Natriuretic Peptide (Pro-BNP), were detected via Luminex^® bead-based multiplex assay (Catalog # LXSAH). A substantial increase in Pro-BNP was detected in the medium of cardiomyocytes treated with ET-1, a known inducer of cardiac hypertrophy. Conversely, Doxorubicin and Verapamil decreased Pro-BNP secretion from cardiomyocytes.

Differentiated cardiomyocytes respond physiologically to Endothelin-1 induced hypertrophy

Cardiomyocytes differentiated from BG01V human embryonic stem cells were treated with Endothelin-1 (ET-1; Catalog # 1160) for 28 hours to induce cardiac hypertrophy. Levels of Pro-Atrial Natriuretic peptide (Pro-ANP) secretion, which is known to be elevated during cardiac hypertrophy, were assessed using ELISA (Catalog # DANP00). As expected, detection of Pro-ANP increased proportionally with the concentration of Endothelin-1 treatment.

Endothelin Induces Expression of Atrial Natriuretic Peptide in Cardiomyocytes Derived from iPSCs

JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (ET-1; Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (Pro-ANP), which is known to be elevated during cardiac hypertrophy, was assessed using (A) Western Blot and (B) ELISA (Human NTProANP Quantikine^® ELISA; Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.

Product Specifications

Molecular Weight

2492.00

Formula

C₁₀₉H₁₅₉O₃₂N₂₅S₅

Storage

Store at -20°C

Purity

≥95% (HPLC)

CAS Number

117399-94-7

PubChem ID

16133807

InChI Key

ZUBDGKVDJUIMQQ-ZTNLKOGPSA-N

SMILES

[H][C@](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC1=CNC=N1)NC(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@]2([H])CSSC[C@]([H])(NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC2=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC2=CC=C(O)C=C2)C(=O)N[C@H](CC2=CC=CC=C2)C(=O)N1)([C@@H](C)CC)C(=O)N[C@@]([H])([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Soluble to 1mg/ml in water

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

Background References

References are publications that support the biological activity of the product.

Xiong Endothelin-1 stimulates leptin production in adipocytes. J.Biol.Chem. 2001 PMID: 11359784
Maguire and Davenport ETA receptor-mediated constrictor responses to endothelin peptides in human blood vessels in vitro. Br.J.Pharmacol. 1995 PMID: 7647976
Matsumoto Endothelin-induced changes of second messengers in cultured human ciliary muscle cells Invest.Ophthalmol.Vis.Sci. 1996 PMID: 8631621
Yanagisawa and Masaski Molecular biology and biochemistry of the endothelins. TiPS 1989 PMID: 2690429
Yanagisawa Primary structure, synthesis, and biological activity of rat endothelin, and endothelium-derived vasoconstrictor peptide. Proc.Natl.Acad.Sci.U.S.A. 1988 PMID: 3045827
Servitja Involvement of ET_A and ET_B receptors in the activation of phospholipase D by endothelins in cultured rat cortical astrocytes. Br.J.Pharmacol. 1998 PMID: 9756390
Inoue The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes. Proc.Natl.Acad.Sci.U.S.A. 1989 PMID: 2649896