Human NT-ProANP Quantikine ELISA Kit

Name: Human NT-ProANP Quantikine ELISA Kit
Brand: R&D Systems
SKU: DANP00
Price: 615 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DANP00

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.146 ng/mL
Assay Range:	0.313 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human NT-ProANP Quantikine ELISA Kit Summary:	The Quantikine^® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.

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Product Specifications for Human NT-ProANP Quantikine ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human NT-ProANP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.71	6.04	11.1	1.81	5.95	11.5
Standard Deviation	0.069	0.224	0.583	0.129	0.426	0.842
CV%	4	3.7	5.3	7.1	7.2	7.3

Recovery

The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	104	87-114
EDTA Plasma (n=4)	102	84-120
Heparin Plasma (n=4)	98	84-111
Serum (n=4)	108	95-122

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human NT-ProANP Quantikine ELISA Kit

	Human NT-ProANP ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Atrial Natriuretic Peptide/ANP

Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.

Item	Value
Entrez Gene IDs	4878 (Human); 230899 (Mouse); 24602 (Rat)
Alternate Names	ANF, ANP, ANPatriopeptin, ATFB6, Atrial Natriuretic Peptide, CDD-ANF, Cardionatrin, NPPA, PND, PNDcardionatrin, atrial natriuretic factor, natriuretic peptide A, natriuretic peptide precursor A, prepronatriodilatin

Citations for Human NT-ProANP Quantikine ELISA Kit (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human NT-ProANP Quantikine ELISA Kit include:

Species: Human
Sample Types: Serum

H Zhang et al. (2019-01-01), Associations among NPPA gene polymorphisms, serum ANP levels, and hypertension in the Chinese Han population J Hum Hypertens, 0(0).
PMID: 31341238

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Product Documents for Human NT-ProANP Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.