Key Product Details
Arg319-Phe541 (Lys417Asn, Leu452Arg, Thr478Lys), with a C-terminal 6-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN).
Scientific Data Images for Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His Protein, CF
Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His-tag Protein Binding Activity.Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His-tag (Catalog # 10905-CV) binds Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) in a functional ELISA.
Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His-tag Protein SDS-PAGE.2 μg/lane of Recombinant Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His-tag Protein (Catalog # 10905-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-37 kDa.
Binding of ACE-2 to SARS-CoV-2 AY.1/AY.2 variant Spike RBD protein by surface plasmon resonance (SPR).Recombinant SARS-CoV-2 AY.1/AY.2 variant Spike RBD protein His-tag (Catalog #10905-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.184 nM and 94.3 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=13.8 nM. (Biacore T200).
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Spike RBD
- Wu, F. et al. (2020) Nature 579:265.
- Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
- Bosch, B.J. et al. (2003) J. Virol. 77:8801.
- Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
- Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
- Li, W. et al. (2003) Nature 426:450.
- Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
- Kannan, S.R. et al. (2021) J. Autoimmun. 124:https://doi.org/10.1016/j.jaut.2021.102715.
Product Specific Notices for Recombinant SARS-CoV-2 AY.1/AY.2 Spike RBD His Protein, CF
For research use only