Product Specifications for Recombinant Rat Tie-2 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived rat Tie-2 protein Ala23-Leu743, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ala23 & Met24
Predicted Molecular Mass
95-108 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Rat Tie-2 His-tag (Catalog # 10458-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His‑tag (Catalog # 623-AN) binds with an ED50 of 0.4-3.6 ng/mL.
Scientific Data Examples for Recombinant Rat Tie-2 His-tag Protein, CF
Recombinant Rat Tie-2 His-tag Protein Binding Activity
When Recombinant Rat Tie-2 His-tag (10458-T2) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED50 of 0.4-3.6 ng/mL.
Recombinant Rat Tie-2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Rat Tie-2 His-tag Protein (Catalog # 10458-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-108 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Tie-2, also known as Tek, is a 145 kDa, type I transmembrane glycoprotein receptor tyrosine kinase that is a receptor for angiopoietins (1). The 1120 amino acid (aa) rat Tie-2 precursor contains an 18 aa signal sequence, a 723 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 354 aa cytoplasmic tail (2). The ECD contains two C2 Ig-like domains, three EGF-like motifs, and three fibronectin type III repeats. The cytoplasmic region has a split tyrosine kinase domain and presumably autophosphorylates as a ligand-induced homodimer (3). Rat Tie-2 ECD shares 96%, 90% and 89% aa identity with mouse, human and bovine Tie-2, respectively, and 47% aa identity with rat Tie-1 ECD. Cells known to express Tie-2 include embryonic and adult endothelial cells, hematopoietic stem cells and a circulating population of proangiogenic Tie-2 expressing monocytes (TEM) (4-7). A soluble form of Tie-2, most likely the result of proteolytic cleavage, is found in serum (8). The four angiopoietins are ligands of Tie-2. Ang-1 and Ang-4 are Tie-2 activators, while Ang-2 and Ang-3 can be activators or inhibitors, depending on context (1, 9). Tie-2 is said to be important for maintaining vascular integrity. It mediates endothelial cell-smooth muscle cell communication, and inhibits endothelial cell apoptosis, thus maintaining endothelial cell survival (10-12). It is also absolutely required for embryonic development of the endocardium (3, 10, 13). While not essential for embryonic hematopoiesis, Ang-1 production by osteoblasts promotes quiescence of Tie-2-expressing bone marrow stem cells. This quiescence is critical for maintaining an ongoing hematopoietic capability (12, 14, 15).
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Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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