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Recombinant Rat Tie-2 His-tag Protein, CF

Catalog # 10458-T2 | R&D Systems, Inc. a Bio-Techne Brand
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Product Specifications


Mouse myeloma cell line, NS0-derived rat Tie-2 protein
Ala23-Leu743, with a C-terminal 6-His tag


>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23 & Met24

Predicted Molecular Mass

82 kDa


95-108 kDa, under reducing conditions


Measured by its binding ability in a functional ELISA.
When Recombinant Rat Tie-2 His-tag (Catalog # 10458-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His‑tag (Catalog # 623-AN) binds with an ED50 of 0.4-3.6 ng/mL.

Scientific Data Images for Recombinant Rat Tie-2 His-tag Protein, CF

Recombinant Rat Tie-2 His-tag Protein Binding Activity

Recombinant Rat Tie-2 His-tag Protein Binding Activity

When Recombinant Rat Tie-2 His-tag (10458-T2) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED50 of 0.4-3.6 ng/mL.
Recombinant Rat Tie-2 His-tag Protein SDS-PAGE

Recombinant Rat Tie-2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Rat Tie-2 His-tag Protein (Catalog # 10458-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-108 kDa.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-2, also known as Tek, is a 145 kDa, type I transmembrane glycoprotein receptor tyrosine kinase that is a receptor for angiopoietins (1). The 1120 amino acid (aa) rat Tie-2 precursor contains an 18 aa signal sequence, a 723 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 354 aa cytoplasmic tail (2). The ECD contains two C2 Ig-like domains, three EGF-like motifs, and three fibronectin type III repeats. The cytoplasmic region has a split tyrosine kinase domain and presumably autophosphorylates as a ligand-induced homodimer (3). Rat Tie-2 ECD shares 96%, 90% and 89% aa identity with mouse, human and bovine Tie-2, respectively, and 47% aa identity with rat Tie-1 ECD. Cells known to express Tie-2 include embryonic and adult endothelial cells, hematopoietic stem cells and a circulating population of proangiogenic Tie-2 expressing monocytes (TEM) (4-7). A soluble form of Tie-2, most likely the result of proteolytic cleavage, is found in serum (8). The four angiopoietins are ligands of Tie-2. Ang-1 and Ang-4 are Tie-2 activators, while Ang-2 and Ang-3 can be activators or inhibitors, depending on context (1, 9). Tie-2 is said to be important for maintaining vascular integrity. It mediates endothelial cell-smooth muscle cell communication, and inhibits endothelial cell apoptosis, thus maintaining endothelial cell survival (10-12). It is also absolutely required for embryonic development of the endocardium (3, 10, 13). While not essential for embryonic hematopoiesis, Ang-1 production by osteoblasts promotes quiescence of Tie-2-expressing bone marrow stem cells. This quiescence is critical for maintaining an ongoing hematopoietic capability (12, 14, 15).


  1. Eklund, L. and B. R. Olsen (2006) Exp. Cell Res. 312:630.
  2. Maisonpierre, P.C. et al. (1993) Oncogene 8:1631.
  3. Vikkula, M. et al. (1996) Cell 87:1181.
  4. Asahara, T. et al. (1997) Science 275:964.
  5. Dallabrida, S.M. et al. (2003) Biochem. Biophys. Res. Commun. 311:563.
  6. Takakura, N. et al. (1998) Immunity 9:677.
  7. DePalma, M. et al. (2005) Cancer Cell 8:211.
  8. Reusch, P. et al. (2001) Angiogenesis 4:123.
  9. Lee, H.J. et al. (2004) FASEB J. 18:1200.
  10. Jones, N. et al. (2001) EMBO Rep. 2:438.
  11. Wong, A. L. et al. (1997) Circ. Res. 81:567.
  12. Hamaguchi, I. et al. (2006) Blood 107:1207.
  13. Puri, M.C. et al. (1999) Development 126:4569.
  14. Puri, M.C. and A. Bernstein (2003) Proc. Natl. Acad. Sci. USA 100:12753.
  15. Arai, F. et al. (2004) Cell 118:149.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Gene Symbol



Product Documents for Recombinant Rat Tie-2 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Tie-2 His-tag Protein, CF

For research use only

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