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Recombinant Rat B7-2/CD86 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1340-B2

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1340-B2-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat B7-2/CD86 protein
Rat B7-2
(Val29-Ile250)
Accession # O35531
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val29

Predicted Molecular Mass

52 kDa (monomer)

SDS-PAGE

80-100 kDa, reducing conditions

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.
The ED50 for this effect is 0.075-0.3 µg/mL in the presence of PHA.

Formulation, Preparation and Storage

1340-B2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-2/CD86

B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Mature rat B7-2 consists of a 222 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 19 aa transmembrane segment, and a 46 aa cytoplasmic tail (3). Within the ECD, rat B7-2 shares 59% and 81% aa sequence identity with human and mouse B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (4-7), as well as on vascular endothelial cells (8). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co‑stimulation (4, 5, 7, 9‑12). B7‑1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (4, 5, 7, 9‑11). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (13). B7-2 is expressed earlier than B7-1 following APC activation (6), and both proteins bind with higher affinity to CTLA-4 than to CD28 (11). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (14). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (15). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (16).

References

  1. Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Maeda, K. et al. (1997) Int. Immunol. 9:993.
  4. Azuma, M. et al. (1993) Nature 366:76.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054.
  7. Hathcock, K.S. et al. (1993) Science 262:905.
  8. Seino, K. et al. (1995) Int. Immunol. 7:1331.
  9. Freeman, G.J. et al. (1993) Science 262:909.
  10. Chen, C. et al. (1994) J. Immunol. 152:4929.
  11. Lanier, L.L. et al. (1995) J. Immunol. 154:97.
  12. Rudd, C.E. et al. (2009) Immunol. Rev. 229:12.
  13. Wing, K. et al. (2011) Trends Immunol. 32:428.
  14. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
  15. Kuchroo, V.K. et al. (1995) Cell 80:707.
  16. Short, J.J. et al. (2006) Virus Res. 122:144.

Alternate Names

B72, CD86

Entrez Gene IDs

942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)

Gene Symbol

CD86

UniProt

Additional B7-2/CD86 Products

Product Documents for Recombinant Rat B7-2/CD86 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat B7-2/CD86 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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